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Articles by Essam S. Soliman
Total Records ( 4 ) for Essam S. Soliman
  Essam S. Soliman , Mohamed A.A. Sobeih , Z.H. Ahmad , M.M. Hussein , H. Abdel-Latiff and A.A. Moneim
  A total of 1664 environmental samples (litter, water, swabs and air) were collected from commercial broiler farms located in Ismailia and Zagazig Governorates, Egypt. The bacterial and Fungal isolates that were identified included: Citrobacter Sp, E. coli, Klebsiella oxytoca, Proteus vulgaris, Pseudomonas aureuginosa, Salmonella Sp, Shigella Sp, Staphylococcus aureus, Streptococcus fecalis and Streptococcus pneumonie, Yeast Sp, Pencillium Sp, Aspergillus niger, Aspegillus flavus, Aspergillus nidulans, Mucour and Candida albicans Prevalence and frequencies of the microorganisms were calculated to detect the most predominant microorganisms. Litter samples showed that Pseudomonas areuginosa (24%) and Yeast Sp (37.5%) predominated in closed houses; Klebsiella oxytoca (12%) and Penicillium Sp (28.57%) predominated in open houses in winter, Klebsiella oxytoca (33.33%) and Aspergillus nidulans (22.73%) predominated in closed houses; E. coli (50%) and Penicillium Sp (21.40%) in open houses in spring, Shigella Sp (34.5%); (47.62%) and Aspergillus niger (26.92%); (8.69%) predominated in closed and open houses respectively in summer, Klebsiella oxytoca (55.56%) and Aspergillus niger (12%) predominated in closed houses; Klebsiella oxytoca (41.67%) and Candida albicans (59.1%) predominated in open houses in autumn. Water samples showed that E. coli (39.47%) and Candida albicans (50%) predominated in closed houses; E. coli (60.97%) and Penicillium Sp (60%) predominated in open houses in winter, E. coli (67.57%) and Penicillium Sp (37.5%) predominated in closed houses; E. coli (89.29%) and Aspergillus nidulans (15.83%) predominated in open houses in spring, Shigella Sp (42.55%) and Penicillium Sp (37.5%) predominated in closed houses; Shigella Sp (36.67%) and Yeast Sp (66.6%) predominated in open houses in summer, Klebsiella oxytoca (36.59%) and Candida albicans (33.3%) predominated in closed houses; Klebsiella oxytoca (47.22%) and Candida albicans (47.62%) predominated in open houses in autumn. Swab samples showed that Pseudomonas areuginosa (62.5%) and Penicillium Sp (29.41%) predominated in closed houses; Pseudomonas aureuginosa (47.06%) and Candida albicans (17.64%) predominated in open houses in winter, Klebsiella oxytoca (36.49%) and Aspergillus flavus (43.48%) predominated in closed samples; E. coli (43.48%) and Aspergillus flavus (46.51%) predominated in open houses in spring, Klebsiella oxytoca (28.98%) and Penicillium Sp (34.48%) predominated in closed houses; E. coli (35.17%) and Aspergillus niger (35.14%) predominated in open houses in summer, Pseudomonas areuginosa (31.75%); (42.31%) and Canidada albicans (40.32%); (61.4%) predominated in closed and open houses respectively in autumn. Air samples showed that staphylococcus aureus (51.72%-45.45%, 52%-56.17%, 59.52-69.44 and 48.78-75%) was predominating in closed and open houses respectively in winter, spring, summer and autumn respectively, while the fungal growth showed that Aspergillus niger (66.6%) predominated in closed houses in winter, Aspergillus niger (100%) predominated in closed houses; Aspergillus favlus (100%) predominated in open houses in spring, Aspergillus niger (100%) predominated in closed houses in summer, Aspergillus niger (100%) predominated in closed and open houses in autumn.
  Essam S. Soliman , Mohamed A.A. Sobeih , Z.H. Ahmad , M.M. Hussein and H.A.A. Moneim
  Five disinfectants [TH4® (combination of quaternary ammonium compounds and gluteraldhyde), Microzal® (combination of quaternary ammonium compounds and gluteraldhyde), Incospect IC 22XA (combination of quaternary ammonium compounds, gluteraldhyde and formalin), Povidone Iodine® (iodophore) and Formalin® (commercial formaldehyde 37%) were tested in a laboratory trials against four bacterial isolates (Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa) at concentration of (~105) isolated during epidemiological surveillance. The trials were carried in the presence and absence of organic matter (dried yeast 5%) using MIC use-dilution test. Minutely samples were collected for the bacterial counts. In the absence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 5, 5, 10 and 5 min (p<0.0001) respectively, Microzal® and Incospect IC 22XA achieved the 100% killing efficacy against the four Micro-organisms after 5 min (p<0.0001), Povidone Iodine® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively and Formalin® achieved the 100% killing efficacy after 5, 5, 5 and 20 min (p<0.0001) respectively. In the presence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 10, 5, 20 and 20 min (p<0.0001) respectively, Microzal® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively, Incospect IC 22XA achieved the 100% killing efficacy after 5, 5, 10 and 5 min (p<0.0001) respectively, Povidone Iodine® achieved the 100% killing efficacy against the four micro-organisms after 30 min (p<0.0001) and Formalin® achieved the 100% killing efficacy after 30, 5, 20 and 20 min (p<0.0001) respectively. The results revealed that quaternary ammonium-gluteraldhyde combination (TH4®, Microzal® and Incospect IC 22XA) although they are not proven to be environmentally safe; they are the most powerful disinfectants because of the synergistic action of the quaternary ammonium and gluteraldhyde bases.
  Essam S. Soliman , Ensaf G. Taha , M.A.A. Sobieh and P.G. Reddy
  Combined effects of temperature, relative humidity and litter pH in the presence or absence of organic matter on the survival of S. typhimurium over time was studied. The litter (L: 30 cm x W: 25 cm x D: 6 cm aluminum trays filled with wood shavings) was inoculated with S. typhimurium at initial concentration of 4.8 x 107CFU/ml, then litter trays were placed in a room with microclimate similar to that of a naturally ventilated poultry house. The periodical measurement of S. typhimurium population in poultry litter in relation to the ambient environmental conditions revealed that: in the absence of organic matter; there was a non-significant (p<0.99) negative correlation (-0.07 at confidence level 95%) between ambient temperature and survival of S. typhimurium, a non-significant (p<0.53) positive correlation (+0.04 at confidence level 95%) between relative humidity and survival of S. typhimurium population and a highly significant (p<0.005) positive correlation (+0.67 at confidence level 95%) between litter pH and survival. In the presence of organic matter, there was a non-significant (p<0.55) negative correlation (-0.22 at confidence level 95%) between ambient temperature and survival, a highly significant (p<0.0001) negative correlation (-0.12 at confidence level 95%) between relative humidity and survival and a significant (p<0.05) positive correlation (+0.48 at confidence level 95%) between litter pH and survival. The study suggested that increased litter pH and relative humidity rather than temperature presented a great influence on the increased survival of S. typhimurium. New management practice that will reduce litter pH and relative humidity should be considered in the control plans of Salmonellosis in poultry farms.
  Essam S. Soliman , Mohamed A.A. Sobeih , Z.H. Ahmad , M.M. Hussein and H. Abdel-Latiff
  Three different concentrations of five commercial disinfectants [TH4® 2, 1 and 0.5% (combination of quaternary ammonium compounds and gluteraldhyde), Microzal® 2, 1 and 0.5% (combination of quaternary ammonium compounds and gluteraldhyde), Incospect IC 22XA 2, 0.5 and 0.25% (combination of quaternary ammonium compounds, gluteraldhyde and formalin), Povidone Iodine® 5, 7.5 and 10% (iodophore) and Formalin® 3.7, 2.5 and 1.8% (commercial formaldehyde 37%)] were tested in a laboratory trials against two fungal isolates (Aspergillus niger and Candida albicans) at concentration of (~105) isolated during epidemiological surveillance in broilers farms. The trials were carried in the presence and absence of organic matter (dried yeast 5%) using MIC use-dilution test. Minutely samples were collected for the fungal counts. In the absence of organic matter, TH4® 2, 1 and 0.5% achieved 100% efficacy after 5, 5 and 10 min (p<0.001) respectively against Aspergillus niger and Candida albicans. Microzal® 2, 1 and 0.5% achieved 100% efficacy after 5, 10 and 20 min (p<0.001) respectively against Aspergillus niger and after 5 min (p<0.001) against Candida albicans. Incospect IC 22XA 2%, 0.5% and 0.25% achieved 100% efficacy after 5 min (p<0.001) against Aspergillus niger and after 5, 5 and 10 min (p<0.001) respectively against Candida albicans. Povidone Iodine® 10, 7.5 and 5% achieved 100% efficacy after 5, 5 and 20 min (p<0.001) respectively against Aspergillus niger and after 5, 5 and 20 min (p<0.001) respectively against Candida albicans. Formalin® 3.7, 2.5 and 1.8% achieved 100% efficacy after 5, 10 and 10 min (p<0.001) respectively against Aspergillus niger and after 5, 5 and 10 min (p<0.001) respectively against Candida albicans. In the presence of organic matter, TH4® 2, 1 and 0.5% achieved 100% efficacy after 5, 10 and 20 min (p<0.001) respectively against Aspergillus niger and after 5, 5 and 20 min (p<0.001) respectively against Candida albicans. Microzal® 2, 1 and 0.5% achieved 100% efficacy after 5, 20 and 20 min (p<0.001) respectively against Aspergillus niger and after 5, 10 and 10 min (p<0.001) respectively against Candida albicans. Incospect IC 22XA 2, 0.5 and 0.25% achieved 100% efficacy after 5 min (p<0.001) against Aspergillus niger and after 5, 5 and 10 min (p<0.001) respectively against Candida albicans. Povidone Iodine® 10, 7.5 and 5% achieved 100% efficacy after 30 min (p<0.001) against Aspergillus niger and Candida albicans. Formalin® 3.7, 2.5 and 1.8% achieved 100% efficacy after 5, 10 and 20 min (p<0.001) respectively against Aspergillus niger and after 5, 5 and 20 min (p<0.001) respectively against Candida albicans. The results revealed that Incospect IC 22XA was the superior in the efficacy and the fastest disinfectant to achieve the 100% killing against the two organisms.
 
 
 
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