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Articles by Hamid Rashid
Total Records ( 9 ) for Hamid Rashid
  Muhammad Azhar Bashir , Hamid Rashid and Muhammad Akbar Anjum
  Nodal segments of six promising strains of jojoba i.e., PKJ-1 to PKJ-6 were cultured in vitro on solidified MS medium supplemented with BA and Kinetin, alone or in combinations at different concentrations to initiate shoot growth. Different growth parameters regarding shoot initiation and growth were recorded during the experimental period of three months. The study revealed that BA (alone) was better than Kinetin (alone) or BA + Kinetin combinations. The lowest concentration of BA (1.25 mg L-1) proved more effective for many shoot parameters and PKJ-3 strain was the most responsive to cytokinins alone or in combination. Some explants also developed callus at the highest level (5.0 mg L-1) of BA (alone) or in combinations of BA with Kinetin. Many explants were vitrified at the highest level (5.0 mg L-1) of Kinetin (alone).
  Zubeda Chaudary , Imran Feroz , Waseem Ahmed , Hamid Rashid , Bushra Mirza and Azra Quraishi
  The varietal response of Feston and Nagina was tested using hypocotyl and leaf disc as explant source. The callus formation was achieved on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and on MS medium supplemented with Napthalene Acetic Acid (NAA) and Benzylamino Purine (BAP) from both explant sources. Shoot formation from calli of Feston and Nagina were successfully obtained on different hormonal levels of BAP & NAA on MS medium. Maximums shoot percentages of calli derived from hypocotyl (56.00%) and leaf disc (42.30%) was achieved on MS medium with 0.05mg/l IAA and 4.0mg/l BAP for Feston. For Nagina, maximum shoot percentage was obtained from leaf explant (39.13%) and hypocotyl (32.00%) on similar medium. Shoot regeneration produced 3.137 and 2.353 mean number of shoots/explants for hypocotyl and leaf disc of Feston whereas Nagina responded with 2.187 and 1.783 mean number of shoots/explant for leaf disc and hypocotyl respectively.
  Hamid Rashid , Syed Yassir Abbas Bokhari and Azra Quraishi
  A protocol has been developed for callus induction, regeneration and hygromycin selection of rice (Oryza sativa L. cv. Super Basmati). Mature seeds of rice were used as starting material for callus induction and cultured on Murashige & Skoog medium (MS) and N6 medium having different levels and combinations of 2,4-Dicholro Phenoxy Acetic Acid (2,4-D). 2,4-D @ 2 mg/l gave good response to callus induction. For regeneration, calli were cultured on MS medium having different concentrations of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP). Medium containing NAA @ 1mg/l with BAP 5 mg/l showed best results with regeneration frequency of 45.3%. Hygromycin at the concentration of 50 mg/l proved to be lethal for secutellum-darived calli of Super Basmati.
  Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi
  Effects of different growth regulators on uni-nodal explants of field-grown olive (Olea europaea L.) cv. Leccino were investigated. Single-node cuttings were obtained from 8th bud pair. Disinfection protocols were standardized by using 0.1% mercuric chloride for five min followed by 50% Clorox for 15 min. sterilized cuttings were tested on MS medium supplemented with various plant growth regulators viz. gibberellic acid kinetin benzylaminopurine, and isopentenyladenine. These growth regulators were used singly in different combinations ranging from 1.0 to 8.0 μM l -1 In vitro. GA3 @ 4 μM l -1 was found to be more effective in enhancing shoot elongation. A higher concentration of kinetin, BAP and 2iP induced more callusing and hyperhydrated microshoots. Inclusion of polyvinylpyrrolidone @ 0.2 g/l in media was found to be successful to eliminate phenolic exudates.
  Aish Muhammad , Iqbal Hussain , Muhammad Faheem Akbar and Hamid Rashid
  Multiplication rate of banana plants c.v. Basrai in the field was studied. The results showed that plants raised through culture produced 7.45 suckers plant-1 after 14 months of planting. On the other hand, number of suckers produced by banana plants propagated conventionally were 4015 in the same period of time. The results indicated that tissue culture plants produced 44% more suckers. This enhanced rate of multiplication may overcome the limited sucker production in the field.
  Ihsan Ullah , Hamid Rashid and M. Ramzan Khan
  Hypocotyl segments of rapeseed cultivar "Rainbow" were cultured on B5 medium supplemented with several concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) ranging from 0.0-4.0 mg l-1. Response to callus induction differed according to the levels of hormonal treatments. 2,4-D @1.5 mg l- induced growth of compact, green callus whereas other levels of 2,4-D produced poorly developed calli. Embryogenic calli were transferred to the medium supplemented with different combinations of Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) at levels of 0.0/0.0, 0.1/0.5, 1.0/0.5, 2.5/1.0, 5.0/1.0 and 5.0/2.0 mg l-1, respectively. More than 50% of the calli cultured on the medium supplemented with BAP@ 5.0 mg l-1 with NAA 1.0 mg l-1 regenerated into plantlets.
  Zubeda Chaudhry , Darima Habib , Hamid Rashid and Asfari Sharif Qureshi
  The in vitro response of Roma was tested using hypocotyls and leaf discs as an explant source for callus induction and regeneration. Leaf discs and hypocotyl showed variable response by use of different growth regulators. Formation of calli from leaf discs and hypocotyls were obtained on MS medium with IAA 2 mg l -1, BAP 2 mg l-1, NAA 2 mg l-1 and kinetin 4 mg l-1. Callus formation was 82.5% from hypocotyl and 57% from leaf discs. Maximum percentages of shoot formation from hypocotyl and leaf discs derived calli was 45.8% and 30.8%, respectively on MS medium with IAA 2 mg l-1, BAP 5 mg l1, NAA 2 mg l-1 and kinetin 4 mg l-1.
  Muhammad Farooq , Hamid Rashid , Ihsanullah , Zubeda Chaudhry and Khan Bahadar Marwat
  Three wheat genotypes i.e. Bakhtawar-92, Punjab-96 and Inqilab-91 were tested for their response to callus induction frequency and their subsequent regeneration on a variety of media combinations. Bakhtawar-92 appeared to be the most responsive genotype to callus induction followed by Inqilab-91 and Punjab-96. It also produced significantly higher amount of callus as compared with other genotypes. However, the medium containing 2 mg l-1 2,4-D (2,4-dicholorophenoxy acetic acid) was found to be optimum for callus induction irrespective of the genotypes studied. Regeneration frequency of Bakhtawar-92 was 40% on the medium containing 0.1 mg l-1 IAA (Indole acetic acid) and 2.5 mg l-1 BAP (6-benzyl amino purine). Punjab-96 and Inqilab-91 showed regeneration of 25 and 33% on the medium supplemented with combination of 0.1 mg l-1 IAA and 0.5 mg l-1 BAP. Regenerated plants were evaluated for plant height, maturity and seed set. They had favourable significant differences from the control plants for the most important agronomic traits; plant height, days to maturity and kernels/spike etc.
  Muhammad Ramzan Khan and Hamid Rashid
  A protocol for rapid in vitro propagation of Saccharum officinarum using meristem and axillary buds as explants was developed. Murashige and Skoog (MS) medium supplemented with 20 g l-1 sucrose, 2 g l-1 gelrite and various combination of kinetin (Kn) and gibberrellic acid (GA3) was used as a culture initiation medium. Thirty three percent of the apical buds and 20% of the axillary buds grew into shoots within 15 days on media containing 1 mg l-1 Kn and 0.1 mg l-1 GA3. Transferring the shoots after they were 5cm long on the liquid medium for 15 days led to the maximum multiplication of the shoots. Plantlets with profuse roots were obtained 10 days after the shoots were removed off and planted on the liquid medium containing 60 g l-1 of sucrose. More then 95% of the plantlets survived after one month when they were transplanted in the vermiculite.
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