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Articles by Kingsley C. Anukam
Total Records ( 2 ) for Kingsley C. Anukam
  Kingsley C. Anukam and Tara E Koyama
  A previously characterized biosurfactant-producing Lactobacillus plantarum KCA-1 isolated from the vagina of a healthy premenopausal woman was used in this study. Lactobacillus plantarum KCA-1 was tested for survival in different concentrations (0, 0.05, 0. 1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0) of porcine and oxgall bile extract, incubated at 37°C for 48 h. Acid tolerance of the culture was studied by inoculating the organism (105CFU mL-1) in de Man Rogas Sharpe (MRS) broth at different pH values adjusted with HCl and incubated at 37°C for 48 h. Lactobacillus plantarum strain KCA-1 that produced biosurfactant needed for preventing adhesion of pathogens was tolerant to oxgall bile. There was an increase of 5 log cycles from the initial bacterial inocula (1x105cfu mL-1) to 1x1010cfu mL-1 at 0.05 and 0.1% concentrations of oxgall bile and 4 log cycle increase (1x109cfu mL-1) at 0.5% oxgall concentration. There was a consistent reduction of 1 log cycle of every 0.5% increase of the oxgall for tested concentrations (0.5 to 4.0%). In addition, Lactobacillus plantarum KCA-1 was tolerant at pH 2.5 for 48 h of incubation as there was 2.3 to 3.8 increase in the log cycles. There was a corresponding increase in the number of cells from 1x105 CFU to 1x109 CFU mL-1 at pH 4.5. The tested potential probiotic strain Lactobacillus plantarum KCA-1 exhibited a satisfactory degree of acid and bile tolerance. The findings suggests that the strain may be a promising candidate for use as a dietary adjunct, but more studies are needed.
  Kingsley C. Anukam and Gregor Reid
  The search for Lactobacilli of African origin with probiotic properties formed the basis of the study. Two hundred and forty one premenopausal healthy (as defined by having no symptoms of vaginal infections and are HIV negative) Nigerian women provided vaginal swabs. The swabs were cultured on de Man Rogosa Sharpe (MRS) agar (pH 5.5) and incubated anaerobically at 37°C for 48 h. Microbial DNA was extracted from the colonies and directly from the swabs, amplified using Polymerase Chain Reaction (PCR) with Lactobacillus primers and processed by Denaturing Gradient Gel Electrophoresis (DGGE). DGGE bands were excised, re-amplified, purified, V2-V3 region of 16S rRNA gene sequenced with 3730xl ABI prism BigDye Terminator and sequence identification was by BLAST. Lactobacillus strains were tested for probiotic properties (H2O2, Pathogen inhibition and Biosurfactant production). Out of 241 swab samples cultured on MRS agar, only 24 (10%) samples had growth of Lactobacillus species at pH 4.5. Lactobacillus gasseri was isolated from 6 (3.3%)samples, followed by L. plantarum (2.4%), L. vaginalis (1.6%), L. fermentum (0.8%), L. crispatus (0.8%) and L. rhamnosus (0.8%). Two species, each of Lactobacillus plantarum and L. fermentum produced huge amount of biosurfactants. In addition they produced H2O2, inhibited the growth of intestinal and urogenital pathogens. The study presents a new understanding by culture method showing lactobacilli composition of the vagina of some Nigerian women. Two Lactobacillus species exhibited probiotic characteristics and clinical studies with these strains are now planned following investigation of the genomic regions encoding probiotic functionality.
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