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Articles by M. Miraboulfathi
Total Records ( 2 ) for M. Miraboulfathi
  A. Khanafari , H. Soudi and M. Miraboulfathi
  The potent mycotoxin aflatoxin B1 is a secondary metabolite of Aspergillus fungi that grow, on a variety of food and feed commodities at any stage during growth, harvest, storage and transportation. The occurrence of aflatoxin contamination is global, with severe problems especially prevalent in developing countries. In present study, corn samples were contaminated with aflatoxin B1 in the concentration of 240 µg/kg. Four trials were inoculated by Lactobacillus plantarum (PTCC 1058). Three control assays were analysed in the same conditions. All the assays were kneaded and incubated for 4-7 days at 37°C. Aflatoxin B1 was determined after extraction by HPLC. Results showed a drastic removal of the mycotoxin with a reduction of 77 % for Aflatoxin B1 by Lactobacillus plantarum. In the Inoculated corns, spore germination of A. flavus was totally inhibited. Results in inoculated spikes showed a high percentage of reduction of aflatoxin after incubation by Lb. plantarum. Gram staining of a sample from inoculated corns and microscopic observation demonstrated that the growth of A. flavus spores was totally inhibited by Lb. plantarum. Fungal spores were surrounded by Lactobacillus plantarum and spores were degraded.
  A. Khanafari , H. Soudi , M. Miraboulfathi and R. Karamei Osboo
  This study assessed the binding of Aflatoxin B1 (AFB1) from contaminated solution by Lactobacillus plantarum PTCC 1058. This strain and AFB1 was incubated (1, 24, 48, 90 h at 37°C) and the amount of unbound AFB1 was quantities by HPLC. The concentration of AFB1 in solution was 0.5 ppm. The stabilities of the bacteria/AFB1 complexes were evaluated by determining the amount of AFB1 remaining bound following three washes. Effect of Incubation time on AFB1 Binding on viable and dead cells were evaluated at 1, 24, 48, 72 and 90 h time points. In 1 h 45% and in 90 h 100% AFB1 was removed from solution by this strain. Autoclaved bacteria didn’t remove AFB1 from solutions efficiently (31% in 1 h and 15% in 24 h). Bacteria in logarithmic growth phase retained 92% of the AFB1 initially bound after three washes. Bacterial binding of AFB1 by this strain was rapid and they were in logarithmic growth phase. These findings further support the ability of specific strains of lactic acid bacteria to bind selected dietary contaminants.
 
 
 
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