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Articles by M.N. Amin
Total Records ( 3 ) for M.N. Amin
  M.Z. Karim , M.N. Amin , M.A.K. Azad , F. Begum , M.M. Rahman , S. Ahmad and R. Alam
  Effects of sucrose, agar and pH on in vitro shoot multiplication of Chrysanthemum were studied. Nodal explant from the ex vitro grown plant was used as the test material. For optimum shoot induction and multiplication in MS medium containing BAP+ sucrose 30 gm l-1, agar 6 gm l-1 and pH 5.5-6.0 proved more effective. The media having 30 gm l-1 sucrose showed the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for number of usable shoots per culture, number of node shoot-1 and average length of shoots and the values were 5.4±0.6, 5.1±0.8 and 5.6±0.4 cm. The highest proliferation response of the explant was observed on MS medium having 6 gm l-1 of agar and the frequency was 100%. Among different level of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5 and 6.0. The results presented here proved to be suitable for the in vitro shoot multiplication of Chrysanthemum morifolium.
  M.N. Amin , M.M. Rahman , K.W. Rahman , R. Ahmed , M.S. Hossain and M.B. Ahmed
  The leaf base explants from the in vitro established shoot cultures were induced to form callus and subsequently to differentiate into shoots on MS medium supplemented with different concentrations and combinations of cytokinins and auxins. The cultured explants produced calli from their cut margins within four weeks of incubation on media supplemented with 0.5-3.0 mg L-1 2,4-D alone and in combination with 0.5-3.0 mg L-1 BA. Maximum number of shoot buds with optimum callus growth was observed on MS medium containing 1.0 mg L-1 BA and 0.1 mg L-1 NAA after six weeks of culture. Rooting was induced in the in vitro regenerated shoots on 2 MS medium with different concentrations and combinations of NAA, IBA or IAA. Rooting performance was best when the microshoots were rooted on 2 MS medium containing 0.2 mg L-1 IBA + 0.2 mg L-1 NAA. The regenerated plantlets were successfully transferred to soil and percentage of their survivability under ex vitro condition was almost ninety.
  M.Z. Karim , M.N. Amin , Asaduzzaman , S. Islam , Faruk Hossin and R. Alam
  An efficient protocol was developed for direct regeneration, multiplication and rooting under in vitro conditions of Chrysanthemum. The frequency of multiple shoot regeneration response was 95 and 91%, for nodal segments and shoot tips, respectively when cultured on the medium containing MS +1.0 mg l-1 BAP. Efficient rooting was achieved on half strength of MS+0.2 mg l-1 IBA. In vitro raised plantlets were transferred to potted soil and finally transferred to the field.
 
 
 
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