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Articles by Mohamad Ramlan Mohamed Salleh
Total Records ( 2 ) for Mohamad Ramlan Mohamed Salleh
  Maizirwan Mel , Maizura Mat Saad , Yumi Zuhanis Hasyun Hashim and Mohamad Ramlan Mohamed Salleh
  Media optimization of RC1 hybridoma cell culture for monoclonal antibody production was carried out in T-Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology (STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaCO3 concentration, respectively. Among the 16 runs tested, Run 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaCO3, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO3 were 0.000069, 0.003968 and 0.342151 (R2 = 0.95476 and R Adj. = 0.88691), respectively.
  Maizirwan Mel , Mohamad Ismail Abdul Karim , Mohamad Ramlan Mohamed Salleh and Noraini Alamin Mohamad Amin
  The aim of this study was to optimize the main media components that affect the production of lactic acid by Lactobacillus rhamnosus fermentation. In the experiment, the two variables (glucose and peptone) were optimized using the Central Composite Design (CCD) method by STATISTICA Software. The optimization studies were successfully carried out in shake flask experiments. The results indicated that the optimum concentration of glucose and peptone for optimum bacterial growth rate and lactic acid production in shake flask were 9.80 and 9.98 g L-1, respectively. The optimum productivity of the lactic acid was 0.630 g g-1 h which correspond to optimum growth rate of the bacteria at 0.341 h-1.
 
 
 
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