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Articles by Mustofa
Total Records ( 7 ) for Mustofa
  Prasetyastuti , Sunarti , Ahmad Hamim Sadewa , Sri Mursiti and Mustofa
  Background and Objective: Type 2 diabetes is characterized by hyperglycemia leads to increased production of reactive oxygen species. There is increasing evidence that active compounds of medicinal plants may be used to reduce oxidative stress. The aims of this study were to investigate anti-diabetic effects of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one derived from Swietenia macrophylla King seed on oxidized LDL, homeostatic model assessment beta-cell function (HOMA-β) and glucagon like peptide 1 (GLP-1) gene expression in diabetic rats. Materials and Methods: A total of 30 rats were used. They were divided into 6 groups as follows: (A) Normal rats, (B) Diabetic rats, (C) Diabetic rats with metformin, (D), (E) and (F) Diabetic rats with 7-Hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chromen-4-one Swietenia macrophylla King seed dose 10, 30 or 90 mg/200 g b.wt., respectively. Oxidized LDL and HOMA-β value were analyzed twice, before and after treatment. The GLP-1 gene expression in intestine was analyzed at the end of study. Data were analyzed by paired t-test and one-way ANOVA followed by Games-Howell test. Results: Administration of three different doses of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds in diabetic rats significantly reduced oxidized LDL and increased HOMA-β value (p<0.001) and also GLP-1 gene expression (p<0.05). Conclusion: These findings demonstrate that administration of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds, improved beta cell function through reducing oxidized LDL and increased GLP-1 expression.
  Syamsudin , Soesanto Tjokrosanto , Subagus Wahyuono , Darmono and Mustofa
  In vitro and in vivo antiplasmodial activities of stem barks extracts of Garcinia parvifolia Miq, medicinal plant traditionally used to treat malaria in Indonesia, have been evaluated. The IC50 value of extracts was determined. Extracts of this plant (n-hexane, ethylacetate and n-buthanol extracts) were tested in vitro for their antiplasmodial activity on 2 strains of Plasmodium falciparum, FCR-3 (chloroquine-resistant strain) and 3D7 (chloroquine-sensitive strain) using a visual method. Cytotoxicity of these extracts were performed on HeLa cells in vitro using MTT method. The in vivo antiplasmodial testing used a standard 4-days test on P. berghei infected mice and acute toxicity testing on mice were also conducted. The IC50 values of the plant extracts were in the range of 4,83-40,10 g mL 1. Significant in vitro antiplasmodial activity and higher selective toxicity were observed by n-hexane extract (IC50 = 4,11 µg mL 1 and C/A = 48,23). The n-hexane extract was the most active in vivo against P. berghei (ED50 = 19,95 mg kg 1BW) and least toxic in mice (LD50= 1060,47 mg kBW 1). These results suggest that n-hexane extract is promising extract for further investigation for new antimalarial agents.
  Dwi Sutiningsih , Mustofa , Tri Baskoro Tunggul Satoto and Edhi Martono
  Background and Objective: Bruceine A is a quassinoid compound isolated from the seeds of Makassar fruit (Brucea javanica L. Merr.) that has a biolarvicidal effect toward Aedes aegypti larvae with a lethal concentration fifty (LC50) and ninety (LC90) of 0.408 and 4.923 ppm, respectively. This study aimed to demonstrate the inhibitory effect of bruceine A on the growth and development of A. aegypti larvae at sublethal concentrations. Materials and Methods: The effect of bruceine A on A. aegypti larvae growth and development was tested using bioassays. Daily counts of the number of larvae that survived or died were taken until all the experimental larvae became either pupae or adults. Dead larvae were assessed for morphological and histological changes. The statistical analysis of growth and development inhibition was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on morphological and histological changes were descriptively analyzed. Results: Inhibitory effects of bruceine A on the growth and development of A. aegypti larvae were seen at concentrations as low as 0.01 ppm. The morphological changes seen in larvae included damage to the gastrointestinal tract manifested as black spots, folded breathing tubes and detached cuticle and setae as well as smaller body sizes compared to the control group. Histological examination of the larvae indicated diffuse necrosis in the gastrointestinal epithelium accompanied by epithelial cell shrinkage, whereas the core was not visible. Conclusion: It is concluded that bruceine A inhibits growth and development of A. aegypti larvae, as shown by cuticle damage and necrosis of gastrointestinal and respiratory epithelial cells.
  Syamsudin , Soesanto Tjokrosonto , Subagus Wahyuono and Mustofa
  The study of in vivo antiplasmodial activity and acute toxicity of the active fraction of Garcinia parvifolia Miq. has been conducted. The fraction was obtained by maceration of n-hexane extract with methanol. A standard 4-day test on P. berghei infected Swiss mice was used to evaluate the in vivo antiplasmodial activity after an oral administration of the fraction in series dose of 25 to 200 mg kg-1 b.wt. once daily for 4 consecutive days. The in vivo antiplasmodial activity was expressed by the dose inhibiting 50% of parasite growth (ED50). Acute toxicity was evaluated in Swiss mice after oral administration of the active fraction in series dose of 2000 to 8000 mg kg-1 b.wt. The acute toxicity was expressed by the dose leading 50% deaths (LD50). The results showed that the active fraction of G. parvifolia Miq. was active against P. berghei in mice with an ED50 of 74.45 mg kg-1 b.wt. day-1. In addition, the active fraction was also relatively safe as expressed by the LD50 of 8000 mg kg-1 b.wt.
  Dwi Sutiningsih , Mustofa , Tri Baskoro Tunggul Satoto and Edhi Martono
  Background and Objectives: Aedes aegypti Linnaeus is a mosquito species that plays an important role as a vector of diseases in tropical and subtropical regions. Aedes aegypti (L.) resistance against temephos and pyrethroid has been widely reported in several countries including Indonesia. This study aimed to examine the neurotoxic mechanism of bruceine A biolarvicide and to determine the inhibitory activity of the enzyme acetylcholinesterase and gene Voltage-Gated Sodium Channel (VGSC) on A. aegypti (L.) larvae. Materials and Methods: Bruceine A was obtained by extraction and isolation of Makassar fruit’s seeds. Test of inhibition of acetylcholinesterase activity was based on Ellman method. Voltage-Gated Sodium Channel gene inhibitor was tested using PCR. The statistical analysis of inhibition of the enzyme acetylcholinesterase was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on behavior response and VGSC gene were descriptively analyzed. Results: Bruceine A could inhibit the action of the enzyme acetylcholinesterase as much as 45.083±0.003%, with symptoms of a neurotoxic that is excitation, convulsions, tremors and paralysis. The result of fragment amplification of VGSC gene and electrophoresis on agarose gel 2% generated in a specific band with the size of the fragment bands of 336 bp on samples of larvae that were still sensitive to bruceine A and cypermethrin. Conclusion: Bruceine A isolated from the seeds of Brucea javanica (L.) Merr. has been demonstrated as biolarvicide against larvae of A. aegypti (L.), through its neurotoxic properties. The mechanism of neurotoxicity is mediated through inhibition of enzyme acetylcholinesterase and VGSC gene.
  Syamsudin , S. Wahyuono , S. Tjokrosonto and Mustofa
  The study of in vivo antiplasmodial activity and acute toxicity of the active fraction of Garcinia parvifolia Miq has been conducted. The fraction was obtained by maceration of n-hexane extract with methanol. A standard 4-day test on P. berghei infected Swiss mice was used to evaluate the in vivo antiplasmodial activity after an oral administration of the fraction in series dose of 25-200 mg kg 1 BW once daily for 4 consecutive days. The in vivo antiplasmodial activity was expressed by the dose inhibiting 50% of parasite growth (ED50). Acute toxicity was evaluated in Swiss mice after oral administration of the active fraction in series dose of 2000-8000 mg kg 1 BW. The acute toxicity was expressed by the dose leading 50% deaths (LD50). The results showed that the active fraction of G. parvifolia Miq was active against P. berghei in mice with an ED50 of 74.45 mg/kg BW/d. In addition, the active fraction was also relatively safe as expressed by the LD50 of 8.000 mg kg 1 BW.
  Budijanto , Singgih Susilo , Mustofa and Hadi Soekamto
  Study area about 2 decades ago is an area that most of the people are poor but in just a decade, remittances make the area turned into a center of diverse economic activities, neighborhood conditions which are arranged and luxurious impression. However how far are the factors capable to reaching into the 7 determinants from the level of achievement of the value-expectancy of household workers in the area of origin. The purposes of research as well as to obtain the level achievements of value-expectancy also want to discover and uncover the factors that affect the level of achievement of the value-expectancy of household workers in the area of origin. This study uses quantitative approach through survey methods. Sample areas are determined by purposive that is districts/villages which have the largest number of household workers. Total sample are 250 household workers were simple randomly selected (simple random sampling). The analysis data is descriptive and analysis statistical of Partial Least Square (PLS). The findings research showed that remittances have a significant effect on the level of achievement of all 7 determinants of value-expectancy. Meaning that remittances have increased prosperity, status, comfort, stimulants, autonomy, affiliation and morality, the household workers in the area of origin. Thus remittances who sent to households of origin area has maximize the actual revenues economic that is expected, social mobility and social status that is achieved, resident satisfaction, affiliation with household and friends and life-style preferences that is achieved.
 
 
 
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