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Articles by Nirmal Mandal
Total Records ( 3 ) for Nirmal Mandal
  Arpita Das , Saikat Gantait and Nirmal Mandal
  To sustain the supply of quality propagules, the present study was carried out to develop a novel protocol for accelerated in vitro mass multiplication in stevia (Stevia rebaudiana Bert.) through multiple shoot induction using shoot tips, nodal segments and axillary bud explants. The earliest bud induction was recorded from the shoot tip explants within 6 days of culture in Murashige and Skoog (MS) medium, in comparison to the other two explants. MS basal medium supplemented with sucrose (30 g L-1), agar (7 g L-1) and kinetin (2 mg L-1) performed best in multiple shoot proliferation, resulting more than 11 multiple shoots from a single shoot tip explant within 35 days of culture. For root induction and elongation, MS medium devoid of plant growth regulator performed most dynamically, where MS medium plus indole-3-acetic acid and 6-benzylaminopurine showed an adverse effect, promoting undesirable callus growth at root zone. In vitro generated propagules were successfully acclimatized in a balanced mixture of sand, soil and farm yard manure (1:1:1 v/v). Peroxidase assay along with ISSR fingerprinting confirmed the genetic clonality of in vitro generated propagules.
  Saikat Gantait and Nirmal Mandal
  Anthurium, with over 108 described genera and 3750 species, is one of the most popular tropicals, highly appreciated for its brightly coloured and long lasting flowers. The exotic Anthurium industry plays a significant role in the global floriculture trade. To overcome the demerits of conventional propagation of Anthurium, plant tissue culture proved to be an influential tool that can complement conventional breeding and accelerate Anthurium development. This review presents a consolidated explanation of in vitro propagation and focuses upon contemporary information on biotechnological advances made in Anthurium.
  Saikat Gantait , Nirmal Mandal and Prakash Kanti Das
  An innovative protocol on accelerated in vitro propagation and acclimatisation was developed in Aloe vera L. Culture was initiated with rhizomatous stem where Murashige and Skoog (MS) medium fortified with 0.5 mg L−1 α-naphthalene acetic acid and 1.5 mg L−1 N6-benzylaminopurine (BAP) promoted earliest shoot induction. Maximum shoot multiplication was achieved in MS medium supplemented with 2.5 mg L−1BAP. The best in vitro rooting was observed in the MS medium with 0.5 mg L−1 indole-3-acetic acid plus 2 g L−1 activated charcoal. The simple acclimatisation process, primarily with a combination of sand and soil (1 : 1 v/v) and finally with a blend of sand, soil and farm yard manure (2 : 1 : 1 v/v), ensured a 98% survival rate. Overall, 192 true-to-type plantlets were achieved from a single explant within 85 days. Morphologically, in vitro generated plants performed better than conventionally propagated plants; nevertheless the similarity in aloin content, gel content and superoxide dismutase activity was corroborated.
 
 
 
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