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Articles by R. Ahmed
Total Records ( 8 ) for R. Ahmed
  A.T.M. Rafiqul Hoque , M.B. Uddin , R. Ahmed and M.K. Hossain
  The paper presents the suppressive effect of different concentration of aqueous leaf extracts of Neem (Azadirachta indica) on some agricultural crops e.g. Cicer arietinum L., Brassica juncea (L.) Czern and Coss; Cucumis sativus L.; Phaseolus mungo L.; Raphanus sativus L.; and Vigna unguiculata (L.) Walp. The experiment was conducted in sterilized petridishes with a photoperiod of 24 hours on an average temperature of 29.50C. The result showed that aqueous leaf extract of Azadirachta indica caused significant inhibitory effect on germination, root and shoot elongation and development of lateral roots of receptor plants. The effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect whereas in some cases the lower concentration showed stimulatory effect. The study also revealed that inhibitory effect was much pronounced in root and lateral root development rather than shoot and germination.
  M.M. Rahman , M.N. Amin and R. Ahmed
  In the present study an efficient tissue culture method for high rate of shoot regeneration was developed for Elaeocarpus robustus. Cotyledonary explants with and without petiolar end were cultured in ½ MS medium supplemented with auxin and cytokinin. Explant with the petiolar/proximal end showed a better response than those without it in respect of shoot initiation. Half strength of MS medium supplemented with 1.0 mg l-1 BA+0.5 mg l-1 2,4-D was found to be best for producing organogenic callus. The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l-1 BA+0.5 mg l-1 Kn+0.1 mg l-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them ½ in MS medium supplemented with 0.2 mg l-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.
  M.M. Rahman , M.N. Amin , H.S. Jahan and R. Ahmed
  Sprouted rhizome buds were collected from the field grown one year old rhizomes and surface sterilized by treating them with 0.1% HgCl2 for 14 min. The dissected rhizome bud explants (ca.1 cm) of turmeric were cultured in MS medium supplemented with different concentrations and combinations of cytokinin and auxin. The best result towards development of multiple shoot from the cultured explants was obtained when MS medium was supplemented with 2.0 mg l-1 of BA. Rooting experiments with half strength of MS medium and various concentrations of NAA, IBA and IAA revealed that 0.1-1.0 mg l-1 of any auxins was found to be effective for rooting. But root forming performance of IBA was proved to be the best among the three auxins tested. Rooted shoots (plantlets) were gradually acclimatized and successfully established in plastic pot containing garden soil under natural condition. About 70% of the transplanted plantlets survived and were eventually established in the field.
  M.N. Amin , M.M. Rahman , K.W. Rahman , R. Ahmed , M.S. Hossain and M.B. Ahmed
  The leaf base explants from the in vitro established shoot cultures were induced to form callus and subsequently to differentiate into shoots on MS medium supplemented with different concentrations and combinations of cytokinins and auxins. The cultured explants produced calli from their cut margins within four weeks of incubation on media supplemented with 0.5-3.0 mg L-1 2,4-D alone and in combination with 0.5-3.0 mg L-1 BA. Maximum number of shoot buds with optimum callus growth was observed on MS medium containing 1.0 mg L-1 BA and 0.1 mg L-1 NAA after six weeks of culture. Rooting was induced in the in vitro regenerated shoots on 2 MS medium with different concentrations and combinations of NAA, IBA or IAA. Rooting performance was best when the microshoots were rooted on 2 MS medium containing 0.2 mg L-1 IBA + 0.2 mg L-1 NAA. The regenerated plantlets were successfully transferred to soil and percentage of their survivability under ex vitro condition was almost ninety.
  A.T.M. Rafiqul Hoque , R. Ahmed , M.B. Uddin and M.K. Hossain
  Allelopathic effect of leaf extracts (different concentration) of Acacia auriculiformis and its possible phytotoxicity was tested in a laboratory experiment by using some agricultural crops e.g. Brassica juncea (L.) Czern & Coss; Phaseolus mungo L.; Raphanus sativus L. and Vigna unguiculata (L.) Walp. and Cicer arietinum L. as a bioassay material. The experiment was conducted in sterilized petri dishes with a photoperiod of 24 hours on an average of 29°C. The effects of the different concentrations of aqueous extracts were compared to distill water (control.). The aqueous extracts caused significant inhibitory effect on germination, root and shoot elongation and development of lateral roots of receptor plants. Bioassays indicated that the inhibitory effect was proportional to the concentrations of the extracts and higher concentration (50-100%) had the stronger inhibitory effect whereas in some cases the lower concentration (10-25%) showed stimulatory effect. The study also revealed that inhibitory effect was much pronounced in root and lateral root development rather than germination and shoot growth.
  M.M. Rahman , M.N. Amin , S. Ahmad and R. Ahmed
  Micropropagation of native-olive (Elaeocarpus robustus Roxb.) has been proved difficult due very slow rooting of the shoot cuttings. The objective of the present investigation was to develop a technique for in vitro fast rooting of the aseptically grown shoots of native-olive. Rooting experiment was conducted on 1/2MS medium with various concentrations and combinations of NAA, IBA and IAA along with or without incubation at higher temperature (30 ° C) in dark condition for initial one week. Root forming performance of IBA was proved to be the best among the three auxins tested. Highest frequency of rooting with maximum number of effective roots was observed on the hormone-free medium in normal growth room condition after treating the microcutting with 0.2 mg L-1 IBA and incubated under 30 ± C in dark for initial one week. Early emergence and fast growth of roots without any malformation were also observed at the same treatment. At this culture condition 85% of the microcuttings produced 5.80 ± 0.15 roots per cutting where average length of roots per cutting was 4.65 ± 0.18 cm. The plantlets originated through the above treatment established themselves under ex vitro condition much quicker than those originated through other treatments.
  M.M. Rahman , M.N. Amin , R. Ahmed , M.A.K. Azad and F. Begum
  Regeneration of multiple shoots via callus induction and organogenesis were achieved in native-olive (Elaeocarpus robustus). Callus induction and shoot buds regeneration were obtained from internode explants of Elaeocarpus robustus on their sufficient medium. The best organic callus was found on modified MS (MMS1) medium supplemented with 0.5 mg L1 BA+0.5 mg L1 2,4-D. Development of adventitious shoots occurred when the calli were subcultured on MMS1 medium supplemented with BA and NAA. Maximum frequency (80%) of calli induced adventitious shoots with highest number of 14.05 ± 1.56 shoots per callus were obtained when the medium was fortified with 1.0 mg L1 BA+0.1 mg L1 NAA. Plantlets developed roots when in vitro developed microcuttings were implanted on modified MS (MMS2) medium with 0.2 mg L1 of IBA. Within six weeks of transfer, 65% rooting was achieved on this medium. Rooted shoots (plantlets) were gradually acclimatized and successfully established under natural condition with about 50% survival rate.
  M.M. Rahman , M.N. Amin , T. Ahamed , S. Ahmad , A. Habib , R. Ahmed , M.B. Ahmed and M.R. Ali
  An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.
 
 
 
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