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Articles by Son Radu
Total Records ( 5 ) for Son Radu
  Reza Khakvar , Kamaruzaman Sijam , Wong Mui Yun , Son Radu and Thong Kwai Lin
  Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various natural habitats such as surface waters and different types of soils. Epidemiological and ecological studies on pathogen distribution in the environment are seriously hindered by the lack of efficient detection method especially when the concentration of the pathogen is either very low or is present in a latent, dormant or non-culturable state. With due attention to importance of R. solanacearum in Malaysia and several irreparable losses that every year caused by this bacterium, this is prompted current study to develop a sensitive PCR-Based method to improve the detection of R. solanacearum in natural sources. We selected the previously reported primers (OLI1;OLI2; Y2; JE2) for their sensitivity and specificity detection of the bacterium in water and soil by a modification of PCR assay.
  Lesley Maurice Bilung , Son Radu , Abdul Rani Bahaman , Raha Abdul Rahim , Suhaimi Napis , Cheah Yoke Kqueen , Chandrika Murugaiah , Yousr Abdul Hadi , Tunung Robin and Mitsuaki Nishibuchi
  Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.
  Raftari Mohammad , Azizi Jalilian Farid , A.S. Abdulamir , Son Radu , Sekawi Zamberi and Fatimah Abu Bakar
  Problem statement: Decontaminating meat surfaces has been the big concern of meat industry. Thus, various intervention strategies have been studied to reduce the level of bacteria on animals’ carcass surfaces. Approach: Mixture of different concentrations 1, 1.5 and 2% of acetic, lactic, propionic and formic acids at 1:1 ratio were spray washed on inoculated meat to evaluate their efficacy in reducing numbers of Staphylococcus aureus on meat tissue at 4±1°C. The beef pieces were decontaminated with hot water and then inoculated with S. aureus which then were spray washed with treatments for 15 sec separately. Results: Spray wash combinations of acetic and formic, lactic and formic and propionic and formic acids reduced the number of S. aureus at a range of 1.18-1.43 log cfu mL-1 more than combinations of acetic and lactic, acetic and propionic and lactic and propionic acids on meat tissue. Increasing the concentration of used acids increased the lethality of treatments as lethal effect of 2% concentration >1.5% concentration >1% concentration. Conclusion: Lactic and formic acids Combination showed the strongest lethal effect on S. aureus among other treatments. Moreover, this study showed that the combination of lactic and formic acids treatment is a feasible, safe, and economical decontamination method which is highly recommended for use rather than other combinations or single organic acids.
  John Tang Yew Huat , Saleha Abdul Aziz , Jalila Abu , Farinazleen Mohamad Ghazali , Tuan Zainazor Tuan Chilek , Noorlis Ahmad , Afriani Sandra , Mitsuaki Nishibuchi and Son Radu
  The aim of this study was to investigate the occurrence of campylobacters in chicken at farms (close-house system and open-house system), slaughtering (conventional slaughterhouse and processing plant) and retail (wet market and supermarket). Campylobacter spp. was not found in cloacal swabs in chickens aged of 4 weeks in farms with close-house system. Campylobacter spp. was found in cloacal swabs (95.0%) in four weeks old chicken in farms with open-house systems. End-slaughtering samples from conventional slaughterhouse and processing plant were contaminated with Campylobacter spp. at 84.0% and 94.0%, respectively. Campylobacter contamination on wet market and supermarket samples with 78.0% and 92.0%, respectively. Close-house system at farm level was able to prevent or delay Campylobacter spp. colonization in chickens but contamination by Campylobacter spp. at retail level was still high. Therefore, monitoring of Campylobacter spp. in chicken products at retail level is crucial to reduce risk of human ingestion of Campylobacter spp. through chicken products.
  Son Radu , Rozila Alias , Gulam Rusul , Samuel Lihan and Ooi Wai Ling
  A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5’-CAATGCGTCT-3’), Gen1-50-09 (5’-AGAGGCGATG-3’) and Gen1-50-10 (5’-CCATTTACGC-3’) as primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate the E. coli O157:H7 isolates into one, six and five RAPD patterns; providing bands ranging in size from 0.25 to 4.0 kb. Our results demonstrate that both plasmid profiling and RAPD-PCR fingerprinting methods are suitable tools for a fast and reliable molecular typing of E. coli O157:H7. The RAPD-PCR method is more sensitive with respect to the individualization of isolates and that RAPD-PCR assay could be a valuable technique for epidemiological studies.
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