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Articles by Syamsudin
Total Records ( 10 ) for Syamsudin
  Syamsudin , Sudjaswadi Wiryowidagdo , Partomuan Simanjuntak and Wan Lelly Heffen
  Problem statement: Propolis samples from tropical zones, such as Java (Indonesia) with its vast biodiversity, have become a subject of increasing scientific and economic attention. The association of the chemical composition of propolis from different geographic regions with cytotoxic activities lead to the identification of active principles, a fundamental tool to achieve standardization of this bee product. Approach: The purpose of this research was evaluate the quality of propolis collected at different places in Java (Indonesia) based on cytotoxic activity. The ethanolic extracts of propolis from different areas in Java were tested for cytotoxicity against tumor cell lines (T47D, MCF-7, Hela, Myeloma and Vero) using MTT assay. Propolis samples were collected from Batang (Central Java), Lawang (East Java) and Sukabumi (West Java). Results: The extract of propolis from Batang showed the most potent activity of T47D and MCF-7 with IC50 34.67±8.3 and 37.8±2.5 μg mL-1. The extract of propolis from Sukabumi showed the most potent activity of Hela cell with IC50 147.34±8.9. However, all propolis extract did not show activity of myeloma and Vero cells. Conclusion: Ethanolics extract of three propolis samples from Batang (Central Java), Lawang (East Java) and Sukabumi (West Java) regions in Java were investigated using GC-MS. From 37 compounds identified, 7 among of them were found for the first time in propolis. This indicated that the secondary metabolite extract of propolis from Batang (Central Java) obtained in the study has antiproliferative activity of breast carcinoma cells (T47D and MCF-7).
  Syamsudin , Partomuan Simanjuntak , Ratna Djamil and Wan Lelly Heffen
  Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.
  Syamsudin , Rita Marleta Dewi and Kusmardi
  Problem statement: Malaria caused by various species of prozoan parasites, Plasmodium. The exact mechanism of humoral immunity in eliminating Plasmodium is not completely understood. However, an antimalarial antibody is strongly suspected to play important role in immunity. The purpose of this research was to evaluate the immunomodulator and antiplasmodial activities of Indonesian propolis extracts. Approach: Thirty male BALB/c mice weighing approximately 25-30 g aged between 6 and 8 weeks old were used for propolis treatment in vivo. The mice infected with 0.1 mL of the P. berghei suspension at a concentration of 107 parasites per mouse on day 0. The drug was administered orally for 4 days from day 0-3 at doses 25, 50 and 100 mg kg-1 BW in the experimental group. The control group was given the solven in equal volume for the same duration. This research utilized not only hypersensitivity reaction which measures the humoral immunity by SRBC-immunized mice but also the activity and capacity of peritoneum macrophage phagocytosis in Plasmodium berghei-infected mice. The parasitaemia number was calculated using blood smear method every day for 4 day after the mice had been infected P. berghei to identify the antiplasmodial activity. Results: The research results revealed that Propolis Hydroalcoholic Solution (PHS) had a strong immunomodulatory activity but weak antiplasmodial activity. Conclusion: It was concluded that PHS showed more immunostimulant activity than antiplasmodial activity, proved by the increase of IgG and the macrophage phagocytosis activity and capacity. The antiplasmodial activity of PHS was due to the mice immunity increase so that they lived longer.
  Syamsudin , Shirly Kumala and Broto Sutaryo
  Crude extracts of some parts of Garcinia parvifolia Miq: root, stem bark, fruit and leaf, can be dissolved in various solvents, such as n-hexane, ethylacetate and methanol. The crude extracts of these plant parts were screened for antiplasmodial, antioxidant, cytotoxic and antibacterial activities. Most extracts show antiplasmodial activity but the extracts of roots and stem bark show strong antiplasmodial activities (IC50 7.88 and 4.11 μg mL-1). All of the extracts show antioxidant activities with IC50 <100 μg mL-1. They all show cytotoxic activity with a value of LC50 <1000 μg mL-1. N-hexane extract of leaf and fruit do not show antibacterial activity, while n-hexane and ethylacetae extracts of stem bark, root and fruit show strong antibacterial activity, especially against Staphyloccocus aureus.
  Syamsudin , Djoko Suhardjanto and Niki Lukviarman
  This study aim is to analyze the role of university governance to organizational commitment. In this study, we employ corporate governance framework to analyze organizational behavior in universities. We conduct a survey which involves 120 respondents contains of dean, rector and vice rector and university academic board from 19 Muhammadiyah universities in Indonesia. The results show that university governance has positive effect on affective and continuance commitment in universities. However, university governance does not have significant effect to normative commitment. The implications of this study are there should be an increasing quality of working responsibility in Muhammadiyah universities and an awareness that research is a part of worship.
  Syamsudin , Soesanto Tjokrosanto , Subagus Wahyuono , Darmono and Mustofa
  In vitro and in vivo antiplasmodial activities of stem barks extracts of Garcinia parvifolia Miq, medicinal plant traditionally used to treat malaria in Indonesia, have been evaluated. The IC50 value of extracts was determined. Extracts of this plant (n-hexane, ethylacetate and n-buthanol extracts) were tested in vitro for their antiplasmodial activity on 2 strains of Plasmodium falciparum, FCR-3 (chloroquine-resistant strain) and 3D7 (chloroquine-sensitive strain) using a visual method. Cytotoxicity of these extracts were performed on HeLa cells in vitro using MTT method. The in vivo antiplasmodial testing used a standard 4-days test on P. berghei infected mice and acute toxicity testing on mice were also conducted. The IC50 values of the plant extracts were in the range of 4,83-40,10 g mL 1. Significant in vitro antiplasmodial activity and higher selective toxicity were observed by n-hexane extract (IC50 = 4,11 µg mL 1 and C/A = 48,23). The n-hexane extract was the most active in vivo against P. berghei (ED50 = 19,95 mg kg 1BW) and least toxic in mice (LD50= 1060,47 mg kBW 1). These results suggest that n-hexane extract is promising extract for further investigation for new antimalarial agents.
  Syamsudin , Soesanto Tjokrosonto , Subagus Wahyuono and Mustofa
  The study of in vivo antiplasmodial activity and acute toxicity of the active fraction of Garcinia parvifolia Miq. has been conducted. The fraction was obtained by maceration of n-hexane extract with methanol. A standard 4-day test on P. berghei infected Swiss mice was used to evaluate the in vivo antiplasmodial activity after an oral administration of the fraction in series dose of 25 to 200 mg kg-1 b.wt. once daily for 4 consecutive days. The in vivo antiplasmodial activity was expressed by the dose inhibiting 50% of parasite growth (ED50). Acute toxicity was evaluated in Swiss mice after oral administration of the active fraction in series dose of 2000 to 8000 mg kg-1 b.wt. The acute toxicity was expressed by the dose leading 50% deaths (LD50). The results showed that the active fraction of G. parvifolia Miq. was active against P. berghei in mice with an ED50 of 74.45 mg kg-1 b.wt. day-1. In addition, the active fraction was also relatively safe as expressed by the LD50 of 8000 mg kg-1 b.wt.
  Syamsudin , S. Wahyuono , S. Tjokrosonto and Mustofa
  The study of in vivo antiplasmodial activity and acute toxicity of the active fraction of Garcinia parvifolia Miq has been conducted. The fraction was obtained by maceration of n-hexane extract with methanol. A standard 4-day test on P. berghei infected Swiss mice was used to evaluate the in vivo antiplasmodial activity after an oral administration of the fraction in series dose of 25-200 mg kg 1 BW once daily for 4 consecutive days. The in vivo antiplasmodial activity was expressed by the dose inhibiting 50% of parasite growth (ED50). Acute toxicity was evaluated in Swiss mice after oral administration of the active fraction in series dose of 2000-8000 mg kg 1 BW. The acute toxicity was expressed by the dose leading 50% deaths (LD50). The results showed that the active fraction of G. parvifolia Miq was active against P. berghei in mice with an ED50 of 74.45 mg/kg BW/d. In addition, the active fraction was also relatively safe as expressed by the LD50 of 8.000 mg kg 1 BW.
  Syamsudin , Inawati and Hendig Winarno
  This is a study of the effect of Inai (Lawsonia inermis Linn) leaves extract on glucose, total cholesterol and triglyceride of blood of mice which was induced by alloxan of 70 mg kg 1 BW. Inai leaves extract was obtained by the percolation of dried inai leaves using 70% ethanol. Sample treatments were done at day of 0, 3, 7 and 14th after the mice underwent the hyperglycaemic condition. The results showed that the feeding of 0.8 g kg 1 BW of inai extract decreased the glucose concentration from 194 mg dL 1 to normal condition after the 14th day. A similar result occured on total cholesterol concentration in which the total cholesterol concentration decreased from 148.9-55.3 mg dL 1 and triglyceride concentration decreased from 225.7-76.9 mg dL 1.
  Syamsudin
  The study aimed at determining the effects of the stem bark extracts of Garcinia parvifolia administered orally to P. berghei infected mice in varied dosages to observe Malondialdehyde (MDA) level, SGOT and SGPT and its relationship with liver cell damage and degree of parasitemia. Thirty mice of Swiss strain used in the experiment were assigned into 5 treatment groups: Group 1 (control group) consisted of P. bergheii infected mice reacted with physiological salt solution; group 2 (normal) consisted of uninfected mice; group 3 consisted infected mice treated with stem bark extracts of G. parvifolia with a dosage of 200 mg/kg BW/day; group 4 consisted of infected mice treated with stem bark extracts of G. parvifolia with a dosage of 400 mg/kg BW/day and group 5 consisted of infected mice treated with stem bark extracts of G. parvifolia with a dosage of 800 mg/kg BW/day. The test preparation was administered orally for 4 days. After 4 days of administration, MDA level was measured in the plasma using a spectrophotometer and three mice were killed to obtain the liver for histopathological purpose. The study found 400 mg kg–1 BW that during malaria infection, MDA production increased and liver damage tended to be milder. Administration of stem bark extract of G. parvifolia in oral way could reduce MDA, SGOT and SGPT level (p<0.05), particularly with a dosage of 400 mg and 800 mg/kg BW/day.
 
 
 
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