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Articles by Taufikurahman
Total Records ( 3 ) for Taufikurahman
  Lida Amalia , Taufikurahman and Sri Nanan B. Widiyanto
  Background: Chromium (Cr) plays a role in the production of Reactive Oxygen Species (ROS) that cause oxidative stress. Oxidative stress is a condition when the amount of ROS, such as H2O2, O‾2 and OH‾ (as a by products in a number of metabolic reactions in the cell organelles) exceeds the amount of antioxidants produced. Antioxidants can be either enzyme or non-enzyme. Antioxidant enzymes are such as catalase and ascorbate peroxidase. In the field, it was found that bananas cv. ambon lumut that can be grown in Cr polluted areas. In this study, it was observed that the level expression of catalase (CAT) and ascorbate peroxidase (APX) genes on banana plantlets cv. ambon lumut to determine the mechanisms of banana plants under Cr stress conditions. Materials and Methods: Banana plantlets were grown in vitro on MS medium with the treatment of Cr at 0, 50, 100, 200 and 400 ppm concentrations and maintained for 6 weeks. Growth parameters were analyzed by ANOVA at significance level (α) of 0.05. Gene expression level can be undertaken with qRT-PCR analysis approach. Results: The growth rate of banana plantlets decreased with increasing Cr concentrations in the growth mediums, especially at 200 and 400 ppm. The level of CAT and APX genes expression were observed in the roots and shoots of banana plantlets. At the root, CAT and APX genes are expressed in all treatment conditions, except in the treatment of 50 ppm. At the shoot, only CAT gene was expressed higher than the control and the highest level of CAT gene expression occurred in the treatment of 200 ppm. The level of CAT and APX genes expression reached the highest level on the treatment of 200 ppm. The level of gene expression of CAT reached more than 9 times compared to the control and APX more than three times. Conclusion: (1) The growth rate of banana plantlets decreased with increasing Cr concentrations in the growth mediums and (2) The level of CAT and APX gene expression in plantlets under Cr stress condition were higher than the control. Gene expression level in the roots is higher than the shoots.
  Ana Widiana , Taufikurahman , S.H. Limin , I. Hernaman and R. Manurung
  Gelam putih (M. cajuputi Powell) in Central Kalimantan, has not been exploited well. In vitro test conducted to evaluate the potential of gelam leaves as cattle feed. The measured variables were NH3, volatile fatty acids (VFA), dry matter Digestibility (DMD) and organic matter Digestibility (DMO). Completely randomized design was made with 5 different treatments and 4 replications, in order to get 20 experimental units. Data were analyzed by analysis of Variance (ANOVA) Followed by Duncan's multiple range test with a significance level of 5%. The results showed that the giving 100% of gelam leaves produced NH3 dan VFA for about 4.01 and 151.25 mM, also DMD and DMO by 58.35 and 52.38%. These results were significant (p<0.05), higher than the grass field, so it can be concluded that the waste of gelam leaves, potentially be used as cattle feed.
  Ana Widiana , Taufikurahman , S.H. Limin , I. Hernaman and R. Manurung
  Distillation process of M. cajuputi Powell leaves to produce cajuput oil, yields a large amount of solid residue as waste materials. In vitro testing was conducted to evaluate the potential of solid residue of M. cajuputi Powell leaves as cattle feed. The measured variables were NH3, volatile fatty acids (VFA), Dry Matter Digestibility (DMD), and Organic Matter Digestibility (DMO). Completely randomized design was made with 5 different treatments and 4 replications, in order to get 20 experimental units. Data was analyzed by analysis of variance (ANOVA), followed by Duncan's multiple range test with a significance level of 5%. The results showed that giving 100% of the solid residue of M. cajuputi Powell leaves produced VFA, DMD and DMO for about 102.25 mM, 59.75% and 42.28% respectively, but with lesser amount of NH3 (2.54mM). These results were significant (P < 0.05) and are higher than the results by using field grass. It can be concluded that the solid residue of M. cajuputi Powell leaves has the potential to be used as cattle feed.
 
 
 
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