Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2017.79.84Punica granatum L.) Juice Improves Liver Damage in Carbon Tetrachloride-induced Rats]]>Prasetyastuti Tanjung LaksonoAdhi TaufiqurrohmanRicky Dewi RahardyantiTantra Anandika FebryantoGandhi Mahmud FauYan HerwiyantiSri Ngadikun Sunarti 32017123Background and Objective: Carbon tetrachloride (CCl4), a carcinogenic compound commonly used in household cleaning agents and spot removers can cause free radical-mediated liver damage. Recent research shows polyphenols in pomegranate (Punica granatum L.), rich in exogenous antioxidants, may protect the liver from hepatotoxic damage. This study aimed to investigate the effect of pomegranate juice on CCl4-induced liver damage in a rat model. Materials and Methods: A total of 25 rats, aged 8 weeks and weighing an average of 200 g were used. They were divided into 5 groups as follows: (1) Normal, (2) Hepatotoxic rats and (3), Hepatotoxic rats with pomegranate juice 0.4 mL/200 g body weight (BW) (4) Hepatotoxic rats with pomegranate juice 0.8 mL/200 g BW and (5) Hepatotoxic rats with pomegranate juice 1.2 mL/200 g BW, respectively. Hepatotoxicity was done by intraperitoneal injection of 0.5 mL1 CCl4 kg1 BW for 5 days. Before and after 2 weeks administration of treatment, blood samples were collected for assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (γ-GT) and malondialdehyde (MDA). At the end of the study, the animals were euthanized and the livers were used for histopathological assessment. Data were analyzed by paired t-test and one-way ANOVA followed by the Games-Howell test. Results: Supplementation with three different doses of pomegranate juices in CCl4-induced rats significantly reduced the serum levels of ALT, AST, ALP, γ-GT and MDA (p<0.05) and pomegranate juice as much as 0.8 and 1.2 mL/200 g BW reduced liver damage in CCl4-induced rats. Conclusion: According to findings of this study supplementation with pomegranate juice can improve liver damage induced by CCl4 in rats.]]>Ayala, A., M.F. Muñoz and S. Argüelles,20142014Adewale, O.B., A.O. Adekeye, C.O. Akintayo, A. Onikanni and S. Sabiu,20144)-induced hepatic damage in experimental Sprague Dawley rats: Antioxidant potential of Xylopia aethiopica.]]>3118123Kalyani, G.A., C.K. Ramesh and V. Krishna,2011Desmodium triquetrum DC.]]>73463466Li, R., W. Guo, Z. Fu, G. Ding, Y. Zou and Z. Wang,2011Radix Paeoniae Rubra aqueous extract against CCl4-induced hepatic damage.]]>1686848693Richhariya, A., A.K. Singh, N. Singh and S.K. Singh,2012Cuscutta reflexa Roxb.]]>2142147Elfalleh, W., N. Tlili, N. Nasri, Y. Yahia and H. Hannachi et al.,2011Punica granatum) fruits.]]>76C707C713Zarfeshany, A., S. Asgary and S.H. Javanmard,20142014Wang, J.Y., C. Zhu, T.W. Qian, H., D.D. Wang, F. Zhang and X. Yin,201594348Kumar, S. and A.K. Pandey,20132013Gil, M.I., F.A. Tomas-Barberan, B. Hess-Pierce, D.M. Holcroft and A.A. Kader,20004845814589Anoosh, E., E. Mojtaba and S. Fatemeh,20102620623Hewawasam, R.P., K.A.P.W. Jayatilaka, C. Pathirana and L.K.B. Mudduwa, 2004Epaltes divaricata extract on carbon tetrachloride induced hepatotoxicity in mice. ]]>1203034Ferreira, E.A., E.F. Gris, K.B. Felipe, J.F.G. Correia, E. Cargnin-Ferreira, D.W. Filho and R.C. Pedrosa,20104-induced hepatic injury in mice of phloroacetophenone from Myrcia multiflora.]]>2010Hermenean, A., T. Mariasiu, I. Navarro-Gonzalez, J. Vegara-Meseguer, E. Miutescu, S. Chakraborty and H. Perez-Sanchez,2017in vivo study and molecular modeling.]]>1316711680Khan, R.A., M.R. Khan and S. Sahreen,20124-induced hepatotoxicity: Protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat.]]>2012Zhen, M.C., Q. Wang, X.H. Huang, L.Q. Cao and X.L. Chen et al.,200718795805Olaleye, M.T., A.E. Amobonye, K. Komolafe and A.C. Akinmoladun,2014Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats.]]>21486492Kaur, G., Z. Jabbar, M. Athar and M.S. Alam,2006Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice.]]>44984993Jurenka, J.S.,2008Punica granatum L.): A review.]]>13128144