Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2017.50.70in vitro Raised Clones and Mother Plant of Celastrus paniculatus-Willd, an Endangered Medicinal Plant]]>SasidharanAnusha Tharayil Koonapra BalachandranMegha Kizhakkepurakkal MadasseryJoseph ElyasKothanam Kuzhiyil 2201792Background and Objective:Celastrus paniculatus-Willd belongs to the family Celastraceae is an endangered medicinal plant in India. It has potential role in primary health care system and various herbal drug formulations. The curative property of this medicinal plant is mainly due to the presence of various bioactive compounds. The present investigation was focused on the phytochemical screening of in vitro raised clones and mother plant in order to ensure the qualitative chemical similarity. Materials and Methods: The preliminary phytochemical screening such as fluorescence analysis of the leaf powder, fluorescence analysis of leaf extracts and various physiochemical properties of both in vitro raised clones and mother plant were done. To ensure the presence of various secondary metabolites TLC analysis was performed. The developed plates were sprayed with Dragendroff reagent for alkaloids, fast blue salt reagent for detection of phenolic group, ninhydrin for aminoacids and biogenic amines, vanillin phosphoric acid for detection of terpenoids. In order to confirm the qualitative chemical similarity of in vitro raised clones high performance liquid chromatography was performed. The spectral comparison of extracts was recorded by WIN CATS (1.3.4 version) software. The results were recorded and expressed as the Mean±SD for all the experiments. Results: The preliminary phytochemical screening of leaf extracts of both in vitro raised clones and mother plant exhibited the same result and there was no remarkable difference. TLC based identification of bioactive constituents revealed the presence of alkaloids, flavanoids, steroids, saponins, tannins, phenols, glycosides and reducing sugars. The HPTLC profile of the methanolic leaf extracts revealed the true to type nature of in vitro raised clones. Conclusion: The study revealed the presence of various bioactive constituents in both in vitro raised clones and mother plant of Celastrus paniculatus. The qualitative chemical comparison was done by TLC and HPTLC analysis.]]>Baker, D.D., M. Chu, U. Oza and V. Rajgarhia,20072412251244Salla, M., I. Fakhoury, N. Saliba, N. Darwiche and H. Gali-Muhtasib,201367468479Gokhale, S.B., Y.A. Kulkarni, A. Gokhale, Yele and S. Pune,2011pp: 21-28pp: 21-28Warrier, P.K., V.P.K. Nambiar and C. Ramankutty,1994Vol. 1-5,Katekhaye, S., S. Duggal and A.P. Singh,2011Celastrus paniculatus.]]>11924Debnath, M., R. Pushpan, H. Kumari and K. Nishteswar,2012Celastrus paniculatus. Willd)-A herbal tranquilizer.]]>2811Tortora, G.J. and B. Derrickson,200711th Edn.,Pages: 820Pages: 820Mosby,20096th Edn.,Pages: 1488Pages: 1488Arora, N. and S. Pandey-Rai,2014Celastrus paniculatus Willd. seeds and antioxidant, anti-inflammatory study of its various solvent extracts.]]>61345351Sharma, P.C., M.B. Yelne and T.J. Dennis,2001Vol. 2,Martin, G., S.P. Geetha, A.V. Raghu, I. Balachandran and P.N. Ravindran,2005In vitro multiplication of Holarrhena pubescens.]]>6111116Chase, Jr.C.R. and R. Pratt,194938324331Lal, D. and N. Singh,2010Celastrus paniculatus Willd-an important medicinal plant under in vitro conditions using nodal segments.]]>65561Gupta, A.K., N. Tandon and M. Sharma,2012Vol. 1-10,Harborne, J.B.,19733rd Edn.,Trease, G.E. and W.C. Evans,200911th Edn.,Sofowora, A., 19932nd Edn.,Khandelwal, K.R.,200819th Edn.,pp: 149-153pp: 149-153Sethi, P.D.,19961st Edn.,Wagner, H. and S. Bladt,19962nd Edn.,Pages: 384Pages: 384Stahl, E.,19692nd Edn.,Pages: 1041Pages: 1041Singhal, A.K., V.S. Bhati and V.K. Singhal,2010Geniosporum prostratum (L.) Benth.]]>11924Krishnamoorthy, K. and P. Subramaniam,2014Solena amplexicaulis (Lam.) Gandhi using GC-MS.]]>2014Dan, S., S.S. Dan, P. Mukhopadhayay and M.K. Mukherjee,1982Polyalthia longifolia and Polyalthia longifolia var. pendula as two distinct species.]]>48350351Shahriar, M., A.N.M. Bahar, M.I. Hossain, S. Akhter, M.A. Haque and M.A. Bhuiyan,2012in vitro antioxidant and cytotoxic activity of five different extracts of Withania somnifera root.]]>2450453Waksmundzka-Hajnos, M., J. Sherma and T. Kowalska,2008Pages: 896Pages: 896Jork, H.,1990Pages: 464Pages: 464Yadav, V., A.K. Singh, V.K. Chaudhari, P.K. Verma, A.K. Singh and S. Rajpoot,2014Celastrus paniculatus Willd.]]>2714Kay, L.A.,1938Pratima, H. and P. Mathad,2011Cajanus cajan L.]]>2181185Ansari, S.H.,20061st Edn.,Johansen, D.A.,1940Pages: 523Pages: 523Kokoski, C.J., R.J. Kokoski and F.J. Slama,195847715717Soni, H., G. Nayak, S.S. Patel, K. Mishra and A.K. Singhai,2011Syzygium cumini Linn.]]>2507509Trivedi, P.C.,2006Pages: 258Pages: 258Padal, S.B., P. Chandrasekhar and Y. Vijayakumar,2013398103Raju, Y.R., P. Yugandhar and N. Savithramma,20146369374Tatiya, A., S. Surana, S. Bhavsar, D. Patil and Y. Patil,2012Eulophia herbacea Lindl. Tubers (Orchidaceae).]]>2S50S55Venkatesh, K.V., K.G. Kumar, K. Pradeepa and S.R.S. Kumar,2013Musa paradisiaca cv. Puttabale and Musa acuminate cv. grand naine.]]>6169172Braga, F.G., M.L.M. Bouzada, R.L. Fabri, M.D.O. Matos, F.O. Moreira, E. Scio and E.S. Coimbra,2007111396402Nwachukwu, E. and H.O. Uzoeto,2010Vitex doniana and Cajanus cajan on some bacteria.]]>23747Gowri, S.S. and K. Vasantha,2010Syzygium cumini (L.)(Myrtaceae) leaves extracts.]]>215691573Nadkarani, A.K.,19763rd Edn., Vol. 1,Pages: 296Pages: 296Kokate, C.K., A.P. Purohit and S.B. Gokhale,20035th Edn.,pp: 1-20pp: 1-20Tandon, N.,2010Vol. 1,Anonymous,20011st Edn., Vol. 1,pp: 142-145pp: 142-145Mukherjee, P.K.,2002Pages: 428Pages: 428Sasidharan, S., Y. Chen, D. Saravaran, K.M. Sundram and L.Y. Latha,20118110Bhattarai, H.D., B. Paudel, S.G. Hong, H.K. Lee and J.H. Yim,200862481484Ansari, S.H.,20051st Edn.,pp: 591-592pp: 591-592Gurdeep, R.C. and K.A. Sham,20041st Edn.,pp: 599-616pp: 599-616Shukla, V.J. and U.B. Bhatt,2001pp: 5-10pp: 5-10Su, X.H., M.L. Zhang, W.H. Zhan, C.H. Huo, Q.W. Shi, Y.C. Gu and H. Kiyota,20096146161Salomi, K.R., S.S. Shafeen, C. Roopesh, Y.C.K. Reddy, L. Sandya, S. Nagarjuna and Y.P. Reddy,2011Celastrus paniculatus.]]>38793Liu, R.H.,200378517S520SBlomhoff, R., M.H. Carlsen, L.F. Andersen and D.R. Jacobs Jr.,200696S52S60Vattem, D.A., R. Ghaedian and K. Shetty,200514120130