Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2017.71.79MuktaShamsunnahar M. HossainSumon M. NasiruddinKhondoker Mofazzal IslamMirza 2201792Background and Objective: Salinity is becoming a serious problem in the world and a widespread soil problem in rice growing countries. The saline area is 3 times larger than land used for agriculture. The conventional methods of plant selection for salt tolerance are difficult because of the large effects of the environment. The main objective of this study was to develop salt-tolerant rice varieties by identifying suitable parents and genetic diversity analysis. Materials and Methods: The study was conducted under Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh. Initially 80 germplasms were used to evaluate the salinity tolerance at seedling stage at glass-house following IRRI standard protocol. Among them, 12 were found as salt tolerant, 13 were found as moderately tolerant, 29 were highly susceptible and 26 were susceptible by phenotypic analysis. Among them, 25 germplasms were used for molecular study, which carry all tolerant variety found in phenotypic study (Hogla, Jamai Naru, Dakhsail, Patnai, Kute Patnai, Holde Gotal, Bazra Muri, Ghunshi, Tal Mugur, Nona Bokhra, Kashrail and FL378), 7 were moderately tolerant, 5 were highly susceptible and 1 was susceptible. These germplasms were characterized by 3 SSR markers which are RM510, RM585 and RM336. Data were analyzed by POPGENE (version 1.31), Power Marker (version 3.25) and NTSYS-PC (version 2.2). Results: The number of alleles/locus ranged from 10-12, with an average number of alleles of 11/locus and PIC values ranged from a low of 0.8533 (RM336) to a high of 0.8940 (RM585). The average gene diversity of overall SSR loci for the 25 genotypes was 0.8885, ranged from 0.9024-0.8672. Unweighted pair group method of arithmetic means (UPGMA) dendrogram constructed from Neis (1972) genetic distance produced five distinct clusters of 25 rice genotypes. FL378 of IRRI was used as check variety. It is confirmed that Holde Gotal, Bazra Muri and Hamai were salt tolerant compared to FL378. Conclusion: This scientific information could be used for solution of suitable parents, development of salt tolerant rice varieties, gene identification for salt tolerance and genetic diversity analysis.]]>Singh, R.K. and B. Mishra,199757431438FAO.,20042004Chakravarthi, B.K. and R. Naravaneni,2006Oryza sativa L.).]]>5684688Gnanesh, A.U., V. Krishna, R.S. Kumar, Venkatesh, S.R.S. Kumar and H.E. Shashidhar,2012Oryza sativa L.]]>50164170Amirjani, M.R.,20103616Haque, S.A.,20063813591365Islam, M.M., M.N.H. Mondol, R.M. Emon, S.N. Begum, S.K. Bhowmik and A.K. Hasan,200754548Haq, T.U., J. Gorham, J. Akhtar, N. Akhtar and K.A. Steele,201037634645Haq, T.U., J. Akhtar, S. Nawaz and R. Ahmad,2009Oryza sativa L.) varieties to salinity stress.]]>4129432956Bhowmik, S.K., S. Titov, M.M. Islam, A. Siddika, S. Sultana and M.H. Haque,20094126131Gregorio, G.B.,19971997Islam, M.M.,2004Oryza sativa L.) at reproductive stage.]]>2004Bhuiyan, M.A.R.,20052005Dhar, P., M. Ashrafuzzaman, S.N. Begum, M.M. Islam and M.M.H. Chowdhury,201224550Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley and M.E. Sorrells,199336181186Nei, M.,1972106283292Mohammad-Nejad, G., A. Arzani, A.M. Rezai, R.K. Singh and G.B. Gregorio,20087730736Aliyu, R.E., A.K. Adamu, S.O. Alonge, S. Muazu and G. Gregorio,2011Oryza sp).]]>2011pp: 328332Herrera, T.G., D.P. Duque, I.P. Almeida, G.T. Nunez, A.J. Pieters, C.P. Martinez and J.M. Tohme,20082008Jayamani, P., S. Negrao, M. Martins, B. Macas and M.M. Oliveira,200747879886Nagaraju, J., M. Kathirvel, R.R. Kumar, E.A. Siddiq and S.E. Hasnain,20029958365841Bhowmik, S.K., M.M. Islam, R.M. Emon, S.N. Begum, A. Siddika and S. Sultana,20071044494454Ashraf, M., H.R. Athar, P.J.C. Harris and T.R. Kwon,20089745110