International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2012.119.129Al-ZubairiAdel S. 4201284Zerumbone (ZER) is a natural compound isolated from Zingiber zerumbet smith, family Zingiberaceae. It has been demonstrated to have an in vitro antiproliferative effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. The aim of our study was to assess the genotoxicity of ZER in CHO cells and using the bacterial mutagenicity Ames assay. The Methyl Thiazol Tetrazolium (MTT) screening assay was carried out to determine the cytotoxicity index (IC50) of ZER. The average IC50 value was 20.8 (±5.1) μM. Two cytogenetic end points were used to investigate the clastogenic effects of ZER, namely Chromosomal Aberrations (CA) assay and Micronucleus (MN) test. The micronucleus test and chromosome aberrations induction assay were performed without any metabolic activation. In the bacterial reverse mutagenicity assay (Ames test), there was no mutational change observed in Salmonella Typhimurium strain TA100 in the presence or absence of S-9 liver metabolic activation system. In contrast, MN induction and the frequency of chromosome aberrations in cultures treated with ZER increased significantly and dose-dependently. Chromatid and whole chromosome breaks/gaps, as well as dicentrics, interchanges, endoreduplications and ring chromosomes were the main types of aberrations induced by ZER. However, higher concentration was found to be toxic.]]>Abdul, A.B.H., A.S. Al-Zubairi, N.D. Tailan, S.I. Abdel-Wahab, Z.N.M. Zain, S. Ruslay and M.M. Syam,2008Zingiber zerumbet in human hela cervical cancer cells.]]>4160168Ames, B.N.,198322112561264Ames, B.N., J. McCann and E. Yamasaki,1975Salmonella/mammalian microsome mutagenicity test.]]>31347364Ames, B.N. and L.S. Gold,19971110411052Bustamam, A., S. Ibrahim, N. Devi, M.N. Halkim, A.S. Al-Zubairi and M.M. Syam,2008in vivo animal model for cervical intra-epithelial neoplasia.]]>46170Cortes, F. and N. Pastor,200318105112Craig, W.J.,199970491S499SAzeddine, E., M. Cunha and M. Kirsch-Volders,199813193198Fenech, M.,19932853544Fenech, M.,2000in vitro micronucleus technique.]]>4558195Galloway, S.M.,2000in vitro: Experience in industry and the case for an upper limit on toxicity in the aberration assay.]]>35:191201Gisselsson, D., L. Pattersson, M. Hoglund, M. Heidenblad and L. Gorunova et al.,20009753575362Kim, M., S. Miyamoto, Y. Yasui, T. Oyama, A. Murakami and T. Tanaka,2009124264271Kirsch-Volders, M. and M. Fenech,2001165158Kirsch-Volders, M., T. Sofuni, M. Aardema, S. Albertini and D. Eastmond et al.,2003in vitro micronucleus assay working group.]]>540153163Lo, A.W.I., C.N. Sprung, B. Fouladi, M. Pedram and L. Sabatier et al.,20022248364850Mosmann, T.,1983655563Matsushima, T., M. Hayashi, A. Matsuoka, M. Ishidate Jr. and K.F. Miura et al.,1999in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU).]]>14569580Matsuoka, A., K. Matsuura, H. Sakamoto, M. Hayashi and T. Sofuni,1999in vitro micronucleus test.]]>14385389Murakami, A., D. Takahashi, T. Kinoshita, K. Koshimizu and H.W. Kim et al.,200223795802Murakami, A., T. Tanaka, J.Y. Lee, Y.J. Surh and H.W. Kim et al.,2004110481490OECD.,1997In vitro mammalian chromosome aberration test.]]>1997Pastor, N., M.J. Flores, I. Dominguez, S. Mateos and F. Cortes,2002516113120Rates, S.M.K.,200139603613Snyder, R.D.,1998411235248Snyder, R.D., G.S. Pearl, G. Mandakas, W.N. Choy, F. Goodsaid and I.Y. Rosenblum,200443143158Snyder, R.D., D. Ewing and L.B. Hendry,2006in vitro cytogenetics assays.]]>6094759Sugimoto-Shirasu, K., N.J. Stacey, J. Corsar, K. Roberts and M.C. McCann,2002Arabidopsis.]]>1217821786Sumner, A.T.,1998107486490Sung, B., A. Murakami, B.O. Oyajobi and B.B. Aggarwal,20096914771484Tanaka, T., M. Shimizu, H. Kohno, S.I. Yoshitani and Y. Tsukio et al.,20016919351945Vargas, V.M.F., V.E.P. Motta, A.C. Leitao and J.A.P. Henriques,1990Achyrocline satureoides in prokaryotic organisms.]]>2401318Xian, M., K. Ito, T. Nakazato, T. Shimizu and C.K. Chen et al.,200798118126Al-Zubairi, A.S., A.B. Abdul, M. Yousif, S.I. Abdelwahab, M.M. Elhassan and S. Mohan,2010In vivo and in vitro genotoxic effects of zerumbone.]]>631117Al-Zubairi, A.S., A.B. Abdul and M.M. Syam,201024707712