Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2016.49.55MatsubaraKazuei 32016163Mouse Primordial Germ Cells (PGCs) proliferate and migrate into the Genital Ridges (GRs) and the urogenital complex (UGC) is then constructed from the GR and the mesonephros. However, the mesonephros disappear at an early stage of subsequent embryonic development and the interaction between mesonephros and PGCs are not well understood. In the present study, it has been investigated the need of mesonephros in PGC differentiation by the ectopic transplant. The GR or UGC of female mouse 12.5 days post coitum (dpc) were transplanted into the kidney capsules of female immunodeficient mice. At 4 and 8 weeks after transplantation, the kidney capsules were recovered and the cell types in the grafts were identified by histological analysis. In UGCs graft of 4 weeks after transplantation, the follicles containing with growing oocyte were observed in all recipient kidney. The structures had the characteristics of secondary follicles with zona pellucida and multi-layered granulosa cells. Antibody staining indicated that these follicles differentiated from PGCs and were not apoptotic. But, no follicles were observed at 4 weeks in transplantation of GR without mesonephros. Similarly, follicles were not present at 8 weeks after transplantation of GR or UGC. The mesonephros is necessary for forming the follicles into kidney capsules of recipient immunodeficient mouse. However, the transplantation of UGCs in ectopic kidney capsules should be performed within 4 weeks in order to study the processes of follicular formation/development.]]>Ginsburg, M., M.H. Snow and A. McLaren,1990110521528Mintz, B. and E.S. Russell,1957134207237Tam, P.P.L. and M.H.L. Snow,198164133147Bowles, J. and P. Koopman,200713434013411Baltus, A.E., D.B. Menke, Y.C. Hu, M.L. Goodheart, A.E. Carpenter, D.G. de Rooij and D.C. Page,20063814301434Oulad-Abdelghani, M., P. Bouillet, D. Decimo, A. Gansmuller and S. Heyberger et al.,1996135469477Barnett, K.R., C. Schilling, C.R. Greenfeld, D. Tomic and J.A. Flaws,200612537555Klinger, F.G. and M. de Felici,2002In vitro development of growing oocytes from fetal mouse oocytes: Stage-specific regulation by stem cell factor and granulosa cells.]]>2448595Obata, Y., T. Kono and I. Hatada,2002in vitro.]]>418497497Shen, W., L. Li, Z. Bai, Q. Pan, M. Ding and H. Deng,2007In vitro development of mouse fetal germ cells into mature oocytes.]]>134223231Shen, W., D. Zhang, T. Qing, J. Cheng and Z. Bai et al.,200675615623Mayanagi, T., K. Ito and J. Takahashi,2003125519526Morohaku, K., R. Tanimoto, K. Sasaki, R. Kawahara-Miki and T. Kono et al.,2016in vitro generation of fertile oocytes from mouse primordial germ cells.]]>11390219026Byskov, A.G.S., N.E. Skakkebaek, G. Stafanger and H. Peters,19771237786Byskov, A.G.S.,1974252396397Donnez, J., B. Martinez-Madrid, P. Jadoul, A. van Langendonckt, D. Demylle and M.M. Dolmans,200612519535Qing, T., H. Liu, W. Wei, X. Ye and W. Shen et al.,2008235461Snow, M., S.L. Cox, G. Jenkin, A. Trounson and J. Shaw,200229722272227Stevens, L.C.,197021364382Van Berlo, R.J., B. de Jong, J.W. Oosterhuis, T. Dijkhuizen, J. Buist and A. Dam,19905034163421Choi, S.J. and G.B. Anderson,1998521725Durcova-Hills, G., F. Wianny, J. Merriman, M. Zernicka-Goetz and A. McLaren,2003in vivo and in vitro.]]>71135141Eppig, J.J., K. Wigglesworth and Y. Hirao,20002246068Liu, J., J. van der Elst, R. van den Broecke, F. Dumortier and M. Dhont,2000in vitro culture.]]>6212181223Kaneko, H., K. Kikuchi, J. Noguchi, M. Hosoe and T. Akita,2003in vitro culture.]]>6914881493Senbon, S., A. Ota, M. Tachibana and T. Miyano,200450439444Ozdzenski, W.,197261267278Ohta, H. and T. Wakayama,200450429437Mayanagi, T., R. Kurosawa, K. Ohnuma, A. Ueyama, K. Ito and J. Takahashi,2003125667675Hashimoto, K., M. Noguchi and N. Nakatsuji,199234233238Hayashi, K., S. Ogushi, K. Kurimoto, S. Shimamoto, H. Ohta and M. Saitou,2012in vitro primordial germ cell-like cells in mice.]]>338971975Matoba, S. and A. Ogura,201184631638Bowles, J., D. Knight, C. Smith, D. Wilhelm and J. Richman et al.,2006312596600Koubova, J., D.B. Menke, Q. Zhou, B. Capel, M.D. Griswold and D.C. Page,200610324742479Solter, D. and B.B. Knowles,19787555655569Scholer, H.R.,19917323329Castrillon, D.H., B.J. Quade, T.Y. Wang, C. Quigley and C.P. Crum,2000VASA gene is specifically expressed in the germ cell lineage.]]>9795859590Koubova, J., Y.C. Hu, T. Bhattacharyya, Y.Q.S. Soh and M.E. Gill et al.,20142014