Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1066.1075Escherichia coli O157:H7 among Diarrhoeic HIV/AIDS
Patients in the Eastern Cape Province-South Africa]]>Abong`oB.O. MombaM.N.B. MalakateV.K. MwambakanaJ.N. 82008118This study investigated the prevalence of Escherichia coli O157:H7
in the stool of confirmed and non-confirmed diarrhoeic HIV/AIDS patients.
Escherichia coli O157:H7 was isolated by culture-based and immunomagnetic
separation from three hundred and sixty stool swabs. Identification was
by conventional IMViC, 20E API and molecular techniques. Confirmed and
non-confirmed diarrhoeic HIV/AIDS patients had 56.5% (74/131) and 43.5%
(57/131) respectively of E. coli O157:H7. Molecular results indicated
that the prevalence of E. coli O157:H7 was 12.16% (9/74) and 8.77%
(5/57) from stool swabs of confirmed and non-confirmed diarrhoeic HIV/AIDS
patients. Antimicrobial resistance was higher for E. coli O157:H7
isolates from stools of confirmed HIV/AIDS than it was for non-confirmed
HIV/AIDS patients. Escherichia coli O157:H7 might be a silent cause
of diarrhoea in HIV/AIDS patients. It is recommended that HIV/AIDS patients
with diarrhoea should be screened for E. coli O157:H7 and surveillance
programmes for these bacteria should be established in both urban and
rural areas of South Africa.]]>Bradshaw, D., N. Nadine, L. Ria, G. Pam and J. Jané et al.,20002000Cagney, C., H. Crowley, G. Duffy, J.J. Sheridan and S. O'Brien et al.,2004Escherichia coli O157:H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland.]]>21203212Centers for Disease Control,20042004CSE,20062006Cunin, P., T. Etienne, G. Yves, N. Chouaïbou, B. Raymond, M. Jacques and M.V.M. Paul,1999Escherichia coli O157 emerging in Cameroon?]]>5285290Dixon, W.J. and F.J. Massey,19834th Edn.,pp: 105-107pp: 105-107Dorrington, R.E., L.F. Johnson, D. Bradshaw and T. Daniel,20062006Dynal Product Brochure,2006E coli. For rapid selective enrichment of Escherichia coli O157:H7.]]>2006Eastern Cape Department of Health,20052005Feng, P.,1995Escherichia coli Serotype O157:H7: Novel vehicles of infection and emergence of phenotypic variants.]]>14752Feng, P., P.I. Fields, B. Swaminathan and T.S. Whittam,1996Escherichia coli O157 and other serotypes by using an antiflagellin monoclonal antibody.]]>3428562859Feng, P. and S.R. Monday,2000Escherichia coli serotypes.]]>14333337Fleiss, J.L., A. Tytun and S.H.K. Ury,198036343346Fratamico, P.M., L.K. Bagi and T. Pepe,2000Escherichia coli O157: H7 in foods and bovine feces.]]>6310321037Gannon, V.P., S. D'Souza, T. Graham, R.K. King, K. Rahn and S. Read,1997Escherichia coli strains.]]>35656662Gazzard, B.G.,19882914971505Gray, L.D.,1995Escherichia, Salmonella, Shigella and Yersinia.]]>1995Gunasekera, T.S., R.J. Holland, M.R. Gillings, D.A. Briscoe and D.C. Nethling et al.,2000Paecilomyces lilacinus strains with biocontrol activities against root-knot nematodes.]]>46775783Hayes, C., E. Elliot, E. Krales and G. Downer,200336S106S109Heuvelink, A.E., F.I.A.M. van den Biggelaar, J.T.M. Zwartkruis-Nahuis, R.G. Herbes and R. Huyben et al.,1998Escherichia coli O157 on Dutch dairy farms.]]>3634803487Jorgensen, J.H., J.D. Turnidge and J.A. Washington,19991999pp: 1526-1543pp: 1526-1543Kelly, P., F. Lungu, E. Keane, R. Baggaley, F. Kazembe, J. Pobee and M. Farthing,199631211871191Koyange, L., G. Ollivier, J.J. Muyembe, B. Kebela, M. Gouali and Y. Germani,2004Escherichia coli O157 Kinshasa.]]>2004May, G.R., M.J. Gill, D.L. Church and L.R. Sutherland,199313813881394Mitchell, D.K., J. Snyder and L.K. Pickering,19981998pp: 267-291pp: 267-291Momba, M.N.B., Z. Tyafa, N. Makala, B.M. Brouckaert and C.L. Obi,200632715720Muller, E.E., W.O.K. Grabow and M.M. Ehlers,2003Escherichia coli O157:H7 from environmental and wastewater in South Africa.]]>29427432NCCLS., 19991999Obi, C.L., J. Ramalivhana, M.N.B. Momba, B. Onabulu and J.O. Igumbor et al.,2007610351047Ochman, H., J.G. Lawrence and E.A. Groisman,2000405299304Olorunshola, I.D., S.I. Smith and A.O. Cker,2000108761763Paine, K. and D.R. Flower,20024357365Radu, S., S.A. Mutalib, G. Rusul, Z. Ahmad and T. Morigaki et al.,1998Escherichia coli O157:H7 in beef marketed in Malaysia.]]>6411531156Renter, D.G., J.M. Sargeant, R.D. Oberst and M. Samadpour,2003Escherichia coli O157 strains from range cattle environments.]]>69542547Sein, P.P., A.A. Hoosen, H.H. Crew-Brown, Y. Hoovadia and M.G. Dove et al.,2005208589Shisana, O., T. Rehle, L. Simbayi, W. Parker and K. Zuma et al.,2005pp: 157pp: 157Torres, A.G., X. Zhou and J.B. Kaper,2005Escherichia coli strains to epithelial cells.]]>731829Wang, G., G.C. Clifford and G.R. Frank,2002Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype and components of the type 2 shiga toxin family by Multiplex PCR.]]>4036133619Wong, C.S., S. Jelacic, R.L. Habeeb, S.L. Watkins and P.I. Tarr,2000Escherichia coli O157: H7 infections.]]>34219301936