Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2500.2504Streptococcus agalactiae Isolates from Environmental Samples and Fish Specimens During a Massive Fish Kill in Kuwait Bay]]>QasemJafar A. SameerAl-Zinki SalwaAl-Mouqati SameeAl-Amad AhmedAl-Marzouk FaisalAl-Sharifi 1220081121This study was undertaken to identify and characterize
bacterial isolates obtained simultaneously from dead fish samples during
a massive fish kill in Kuwait Bay and sewage-water samples running into
Kuwait Bay using conventional and molecular techniques. Of the 71 bacterial
isolates studied; 66 were recovered from 7 different fish species and
5 strains were isolated from sewage samples. The species-specific identity
of the isolates was established by phenotypic characteristics and by PCR
amplification of 16S rRNA by using Streptococcus agalactiae-specific
primers. The genotyping of 12 isolates from fish samples and all 5 isolates
from sewage samples was performed by random amplification of polymorphic
DNA (RAPD) analysis. Culture methods identified 44 of 66 (67%) and 4 of
5 (80%) isolates obtained from fish and sewage samples, respectively,
as S. agalactiae. The PCR amplification of 16S rRNA not only confirmed
the results of conventional methods but also resulted in additional identification
of 14 of 66 (21%) isolates obtained from fish samples and the remaining
isolate recovered from sewage sample, as S. agalactiae. A total
of 9 RAPD patterns were observed among the 17 isolates studied; these
RAPD patterns were grouped into three clusters. Interestingly, four of
the isolates recovered from sewage samples produced nearly identical RAPD
band patterns (85-100% similarity) with some of the S. agalactiae
strains isolated from Mullet kidney and brain indicting the possibility
of sewage being the source of infection.]]>Al-Marzouk, A., R. Duremdez, K. Yuasa, S. Al-Zenki, H. Al-Gharabally and B. Munday,2005Liza klunzingeri in Kuwait Bay: The role of Streptococcus agalactiae and the Influence of Temperature.]]>2005pp: 143-153pp: 143-153Baya, A.M., B. Lipiani, F.M. Hetrick, B.S. Robertson and R. Lukacovic et al.,1990Streptococcus sp. with fish mortalities in the Chesapeake Bay and its tributes.]]>13251253Chatellier, S., H. Huet, S. Kenji, A. Rosenau, P. Geslin and R. Quentin,1996Streptococcus agalactiae strains isolated from cerebrospinal fluid of neonates suffering from meningitis.]]>3427412747Chatellier, S., C. Ramanantsoa, P. Harriau, K. Rolland, A. Rosenau and R. Quentin,1997Streptococcus agalactiae strains by random amplified polymorphic DNA analysis.]]>3525732579Duremdez, R., A. Al-Marzouk, J.A. Qasem, A. Al-Harbi and H. Gharabally,2004Streptococcus agalactiae from cultured silver pomfret, Pampus argenteus (Euphrasen), in Kuwait.]]>27307310Ellis, S., M. Kotiw and S.M. Garland,199635279287Evans, J.J., P.H. Klesius, P.M. Gilbert, C. Shoemaker and M. Al Sarawi et al.,2002Streptococcus agalactiae in cultured seabream, Sparus auratus L. and wild mullet, Liza klunzingeri (Day), in Kuwait.]]>25505513Fasola, E., C. Livdahl and P. Ferrieri,19933126162620Kumar, S., K. Tamara and M. Nei,199410189191Lancefield, R.C.,199359571591Martinez, G., J. Harel and M. Gottschalk,2001Streptococcus agalactiae in milk.]]>656872Maslow, J.M., S.M. Arimi and R.D. Arbeit,199317153164Olivares-Fuster, O., P.H. Klesius, J. Evans and C.R. Arias,2008Streptococcus agalactiae isolates from fish.]]>31277283Plumb, J.A.,1994Principal Microbial Diseases.]]>1st Edn.,pp: 231-238pp: 231-238Qasem, J.A., Z.U. Khan, G. Shiji and A.S. Mustafa,2002Mycoplasma pneumoniae in clinical samples.]]>1577782Qasem, J.A., A.S. Mustafa and Z.U. Khan,2008Legionella in clinical specimens and hospital water supply facilities: Molecular detection and genotyping of the isolates.]]>174955Rivas, A.L., R.N. Gonzalez, M. Wiedmann, J.L. Bruce and E.M. Cole et al.,1997Streptococcus agalactiae and Staphylococcus aureus ribotypes recovered from New York dairy herds.]]>58482487Telenti, A., F. Marchesi, M. Balz, F. Bally, E.C. Bottger and T. Bodmer,1993Mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.]]>31175178