Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.740.745Bacillus sp. from Soil]]>Morabbi HeraviKambiz EftekharFereshteh YakhchaliBagher TabandehFatemeh 52008115Lipase production in an indigenous lipolytic Bacillus
sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine
B-Olive oil. The statistical Taguchi model was used to predict the optimum
experimental conditions for bacterial growth and lipase production. Partial
optimization was carried out for selection of salt base, oil, glucose,
NH4Cl and yeast extract concentrations, inoculum density, pH
and agitation. Maximum lipase activity was detected in the cell free supernatants
of cultures grown in a medium containing 10 g L-1 yeast extract,
15 g L-1 NH4Cl, 3 g L-1 K2HPO4,
1 g L-1 KH2PO4, 0.1 g L-1
MgSO4.7H2O, 2 g L-1 glucose, 0.6 mM MgCl2
and 15 ml L-1 olive oil, pH 8.5 at 30 °C for 24 h and low
agitation. The amount of lipase produced in the designed medium was in
agreement with the predicted values by the statistical method. 16S rRNA
cloning and sequencing identified the test organism as Bacillus pumilus.]]>Akatsuka, H., E. Kawai, Y. Imai, N. Sakurai and K. Omori,2003302185192Cho, A.R., S.K. Yoo and E.J. Kim,2000Escherichia coli of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS.]]>186235238Eftekhar, F., J. Fouladi and M. Faghihi,20031183185Gowland, P., M. Kernick and T.K. Sundaram,198748339343Gupta, R., N. Gupta and P. Rathi,200464763781Gupta, N., G. Mehra and R. Gupta,2004Bacillus sp.: Medium optimization by a Plackett-Burman design and by response surface methodology.]]>50361368Jaeger, K.E. and T. Eggert,200213390397Kim, S.S., E.K. Kim and J.S. Rhee,199612718722Kim, H.K., H.J. Choi, M.H. Kim, C.B. Sohn and T.K. Oh,20022+-independent lipase from Bacillus pumilus B26.]]>1583205212Kouker, G. and K.E. Jaeger,198753211213Krishna, S.H., A.P. Sattur and N.G. Karanth,200137916Lee, S.Y. and J. Rhee,1994Pseudomonas putida 3SK in two-phase batch and continuous reactor system.]]>44437443Möller, B., R. Vetter, D. Wilke and B. Foullois,19911991Nthangeni, M.B., H.G. Patterton, A. van Tander, W.P. Vergeer and D. Litthauer,200128705712Pandey, A., S. Benjamin, C.R. Soccol, P. Nigam, N. Krieger and V.T. Soccol,199929119131Prakasham, R.S., C.S. Rao, R.S. Rao, S. Rajesham and D.N. Sarma,2005Bacillus sp. using Taguchi methodology.]]>120133144Rahman, R.N., J.H. Chin, A.B. Salleh and M. Basri,2003269252260Rapp, P.,1995Fusarium oxysporum f. sp. vasinfectum.]]>17832838Rathi, P., V.K. Goswami, V. Sahai and R. Gupta,2002Burkholderia cepacia in a bioreactor.]]>93930936Ruiz, C., F.I. Javier Pastor and P. Diaz,2003Bacillus sp. BP-6 LipA, a ubiquitous lipase among mesophilic Bacillus species.]]>37354359Sambrook, J., D.W. Russell and T. Maniatis,20013rd Edn.,pp: 2100pp: 2100Sharma, R., Y. Chisti and U.C. Banerjee,200119627662Sugihara, A., T. Tani and Y. Tominaga,1991Bacillus sp.]]>109211216Wang, Y., K.C. Srivastava, G.J. Shen and H.Y. Wang,1995Bacillus, strain A30-1 (ATCC 53841).]]>79433438Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J. Lane,1991173697703