Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.204.209Fasciola Spp. From Cattle, North West Iran]]>RouhaniSoheila RaeghiSaber SpotinAdel 42017204Background and Objective: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. Materials and Methods: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm. The ITS1 marker was used to identify different Fasciola and phylogenetic analysis based on ND1 and CO1 sequence data were conducted by maximum likelihood algorithm. Results:Fasciola samples were separated into 2 groups. Almost all specimens had many sperms in the seminal vesicle (spermic fluke) and one fluke did not contain any sperm in the seminal vesicle. The aspermic sample had F. gigantica RFLP pattern with ITS1 gene. Phylogenetic analysis based on NDI and COI sequence data were conducted by maximum likelihood showed a similar topology of the trees obtained particularly for F. hepatica and F. gigantica. Conclusion: This study demonstrated that aspermic Fasciola found in this region of Iran has same genetic structures through the spermic F. gigantica populations in accordance to phylogenetic tree.]]>McManus, D.P. and J.P. Dalton,2006Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica.]]>133S43S61Mas-Coma, S., M.D. Bargues and M.A. Valero,20053512551278Rokni, M.B., W.M. Lotfy, K. Ashrafi and K.D. Murrell,20142014pp: 59-90pp: 59-90Ashrafi, K., M.A. Valero, M. Panova, M.V. Periago, J. Massoud and S. Mas-Coma,2006Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran.]]>55249260Peng, M., M. Ichinomiya, M. Ohtori, M. Ichikawa, T. Shibahara and T. Itagaki,2009Fasciola hepatica, Fasciola gigantica and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA.]]>105809815Terasaki, K., T. Itagaki, T. Shibahara, Y. Noda and N. Moriyama-Gonda,200163735742Chaichanasak, P., M. Ichikawa, P. Sobhon and T. Itagaki,2012Fasciola flukes in Thailand based on their spermatogenesis and nuclear ribosomal DNA and their intraspecific relationships based on mitochondrial DNA.]]>61545549Prasad, P.K., V. Tandon, D. Biswal, L. Goswami and A. Chatterjee,2008Fasciola (Trematoda: Fasciolidae) based on the ribosomal internal transcribed spacer regions.]]>10312471255Periago, M.V., M.A. Valero, M. Panova and S. Mas-Coma,2006Fasciola hepatica and Fasciola gigantica from European and African bovines using a computer image analysis system (CIAS).]]>99368378Itagaki, T., M. Kikawa, K. Sakaguchi, J. Shimo, K. Terasaki, T. Shibahara and K. Fukuda,2005Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA.]]>131679685Ichikawa, M., N. Iwata and T. Itagaki,2010Fasciola species in Japan.]]>7213711374Reaghi, S., A. Haghighi, M.F. Harandi, A. Spotin, K. Arzamani and S. Rouhani,2016Fasciola hepatica and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from the North-East of Iran.]]>910341038Ashrafi, K.,201510306328Mohanta, U.K., M. Ichikawa-Seki, T. Shoriki, K. Katakura and T. Itagaki,2014Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analyses.]]>11324932501Amer, S., A. ElKhatam, S. Zidan, Y. Feng and L. Xiao,2016Fasciola spp. in sheep in Egypt.]]>2016Aryaeipour, M., R. Soheila, B. Mojgan, M. Hadi, K. Bahram, B.R. Mohammad,20144313641371Galavani, H., S. Gholizadeh and K.H. Tappeh,2016Fasciola isolates from west Azerbaijan province Iran based on ITS1 and ITS2 sequence of ribosomal DNA.]]>115264Yakhchali, M., R. Malekzadeh-Viayeh, A. Imani-Baran and K. Mardani,2015Fasciola species isolated from domestic ruminants of Urmia City, Iran.]]>104655Amor, N., A. Halajian, S. Farjallah, P. Merella, K. Said and B.B. Slimane,2011Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences.]]>128196204Ichikawa-Seki, M., P. Ortiz, M. Cabrera, C. Hoban and T. Itagaki,2016Fasciola hepatica from Peru.]]>65171174Constantine, C.C.,200319346348