Research Journal of Immunology1994-79092077-2211Asian Network for Scientific Information10.3923/rji.2017.8.15Gongronema latifolium and Vernonia amygdalina Ethanol Leaf Extract]]>ElijahJoshua Parker ChumaEze YoungUkegbu Chimere AdrainAkabike Chigozie ArinzechukwuIgwe Chinagolum 12017101Background and Objective:Gongronema latifolium and Vernonia amygdalina are medicinal plants that have been used in the treatment of nausea, hypertension, diabetes, stomach ache, high cholesterol level etc. However this research was set out to access comparatively the immuno-stimulatory effect of ethanol leaf extract of Gongronema latifolium and Vernonia amygdalina in cyclophosphamide immuno-suppressed rats. Materials and Methods: Thirty Wistar albino rats of 5 rats per group were used for this study and were divided into 6 groups. Group 1 rats were normal control; group 2 (positive control) rats were administered cyclophosphamide only, at the dose of 50 mg kg1 b.wt. i.p. Group 3 rats were administered low dose (400 mg kg1 b.wt.) of the Vernonia amygdalina leaf extract after cyclophosphamide induction while group 4 rats were administered high dose (600 mg kg1 b.wt.) of the extract after cyclophosphamide induction. Group 5 rats were administered low dose (400 mg kg1 b.wt.) of Gongronema latifolium leaf extract after cyclophosphamide induction while group 6 rats were administered high dose (600 mg kg1 b.wt.) of the extract after cyclophosphamide induction. The results were analyzed using one way analysis of variance (ANOVA) using SPSS version 20. Results: Treatment with Vernonia amygdalina and Gongronema latifolium leaf extract (group 3, 4 and group 5, 6 respectively) showed a significant increase (p<0.05) in SOD activity, glutathione and vitamin C concentrations when compared with group 2 rats treated with cyclophosphamide only. More so treatment with Vernonia amygdalina and Gongronema latifolium leaf extract (group 3, 4 and group 5, 6 respectively) showed a significant decrease (p<0.05) in the liver marker enzymes (ALT, AST and ALP) when compared to group 2 rats treated with cyclophosphamide only, however group 2 rats showed a significant decrease (p<0.05) in total protein concentration and a significant increase (p<0.05) in the total cholesterol concentration and triacylglycerol concentration when compared to the treatment groups. Conclusion: Comparatively, these findings indicate that Vernonia amygdalina may have better antioxidant properties and also exhibited more potency in inhibiting liver damage by lowering the liver marker enzymes compared to Gongronema latifolia.]]>Akpaso, M., I.J. Atangwho, A. Akpantah, V.A. Fischer, A.O. Igiri and E.P. Ebong,2011Vernonia amygdalina (bitter leaf) and Gongronema latifolium (utazi) on the pancreatic β-cells of streptozotocin-induced diabetic rats.]]>12434Nwangwu, S., D.A. Ofusori, S. Josiah, O.F. Amegor, H. Njoya and A.O. Oyaka,2011Vernonia amygdalina on some vital organs in adult wistar rats.]]>97582Nwachukwu, M.I., R.N. Dike, I.O. Nwachukwu, C.N. Ohalete and V.E. Anyanwu,2012Vernonia amygdalina on E. coli.]]>24451Hassan, H.S., M.T. Odunola, M. Garba and M.A. Usman,2007Vernonia amygdalina leaves on the pharmacokinetics of chlorpropamide in man.]]>633439Mohammed, A.A. and A.S. Zakariyau,2012Vernonia amygdalina) as a feed additive in broiler diets.]]>63841Winkelstein, A.,197341273284Young, S.D., M. Whissell, J.C. Noble, P.O. Cano, P.G. Lopez and C.J. Germond,20061230923098ShalizarJalali, A., S. Hassanzadeh and H. Malekinejad,20112266273Nicolini, A., P.A. Mancini, P. Ferrari, L. Anselmi and G. Tartarelli et al.,200458447450Sethi, J. and J. Singh,20152015CIOMS.,19851985Reitman, S. and S. Frankel,1957285663Klein, B., P.A. Read and L.A. Babson,1960In vitro determination of alkaline phosphate in serum or plasma.]]>6269275Jendrassik, L. and P. Grof,19382978289Fridivich, I.,198926477617764Aebi, H.E.,19831983pp: 273-286pp: 273-286Habig, W.H., M.J. Pabst and W.B. Jakoby,197424971307139Baker, E.M., R.E. Hodges, J. Hood, H.E. Sauberlich, S.C. March and J.E. Canham,197114C and 3H-labelled L-ascorbic acid in human scurvy.]]>24444480Allain, C.C., L.S. Poon, C.S.G. Chan, W. Richmond and P.C. Fu,197620470475Trinder, P.,196922158161Slater, R.J.,1986pp: 269pp: 269Sokal, R.R. and F.J. Rohlf,19953rd Edn.,Pages: 887Pages: 887Uboh, F.E., E.E. Edet, I.J. Atangwo and E. David-Oku,2012Gongronema latifolium leaf extract on some kidney enzymes and protein levels in diabetic and non-diabetic rats.]]>2012Ekpo, A., O.A. Eseyin, A.O. Ikpeme and J.E. Edoho,2007Vernonia amygdalina in rats.]]>2193197Iweala, E.E.J., F.O. Uhegbu and O.A. Adesanoye,2013Gongronema latifolium and selenium supplementation in alloxan induced diabetic rats.]]>59197Parker, J.E., O.C. Nwodo, C.Y. Ukegbu and O.O. Prince,2016Abrus precatorius seed methanol extracton paracetamol-induced liver damage in rats.]]>17126134Pal, R., T. Ahmed, V. Kumar, S.G. Suke, A. Ray and B.D. Banerjee,200947723729Ismail, O.O., I.B. Ridwan, O.G. Olaiya, A. Abdulbasit, J.S.O. Rukayat, F.O. Roehan and S.A. Abass,2013Rattus norvegicus].]]>3371378Kedzierska, K., K. Sporniak-Tutak, J. Bober, K. Safranow, M. Olszewska, K. Jakubowska and L. Domanski,2011pp: 3938-3939pp: 3938-3939Rajasekaran, A. and M. Kalaivani,2011in vivo study.]]>13538Nwanjo, H.U., M.C. Okafor and G.O. Oze,2006Gongronema latifolium in streptozotocin-induced diabetic rats.]]>216165Ayoola, G.A., A.D. Folawewo, S.A. Adesegun, O.O. Abioro, A.A. Adepoju-Bello and H.A.B. Coker,20082124128Buher, D. and C. Miranda,20002000Farombi, E.O. and O. Owoeye,2011Vernonia amygdalina and Garcinia biflavonoid.]]>825332555Sugimoto, T., S. Yamashita, M. Ishigami, N. Sakai and K. Hirano et al.,200236157162Ukegbu, C.Y., O. Arome, E. Affiong, A. Ogechukwu and C. Ike,2016Axonopus compressus (P. beauv) in alloxan induced diabetic rats.]]>1215Kanchana, N. and M.A. Sadiq,2011Plumbago zeylanica on paracetamol induced liver toxicity in rats.]]>3151154