Research Journal of Veterinary Sciences1819-19082151-786xScience International10.3923/rjvs.2017.14.19SkarkovaMiroslava HavlicekZdenek 12017101Background and Objective: Many hormones are known to take part in the control of osmoregulatory processes. One of them is oestradiol, but its role in water and electrolyte balance has not been studied much in the vertebrates. This study determined the effects of low doses of oestradiol on plasma sodium, potassium and chloride concentrations in laying hens and effects of serial blood sampling. Materials and Methods: Hens were given an intramuscular injection of 0.6 mg (n = 12) or 0.06 mg (n = 12) oestradiol dipropionate and the control laying hens (n = 12) were given an intramuscular injection of sterile saline. Blood samples were taken from the vena basilica immediately before the oestradiol or saline injection and then 1, 2, 3, 24 and 96 h after treatment to determine the concentration of ions. The variations of ion concentrations were examined by one-way ANOVA. Results: No significant differences of sodium levels were established. Concentrations of potassium decreased significantly 24 h after injection of 0.06 mg oestradiol (p<0.04) and saline treatment (p<0.03) and 96 h after injection of 0.6 mg oestradiol (p<0.03). Concentrations of chloride increased markedly 2 h after injection of 0.06 mg oestradiol (p<0.002) and saline treatment (p<0.006) and 24 h after injection of 0.6 mg oestradiol (p<0.03). A fall in the chloride levels were recorded 96 h after oestradiol (p<0.005) as well as saline treatment (p<0.005). Conclusion: It was concluded that, oestradiol significantly decreased the plasma potassium level 96 h after treatment (p<0.03) and increased the plasma chloride levels 24 h after treatment (p<0.03), but did not affect plasma sodium levels.]]>Hughes, M.R., D.C. Bennett, D.A. Gray, P.J. Sharp and A.M.S. Poon,2006Anas platyrhynchos).]]>149124133Li, J., I.H. Leghari, B. He, W. Zeng, Y. Mi and C. Zhang,201482517524Laurentino, S.S., S. Correia, J.E. Cavaco, P.F. Oliveira, M. de Sousa, A. Barros and S. Socorro,201118161170Wistedt, A., Y. Ridderstrale, H. Wall and L. Holm,20142014Balthazart, J., C.A. Cornil, T.D. Charlier, M. Taziaux and G.F. Ball,2009311323345Jiang, S., L. Cui, C. Shi, X. Ke, J. Luo and J. Hou,2013198252258Ciftci, H.B. and I. Yıldırım,20132013Elkomy, A.E., G. El-Shaarrawi, E. El-Ansary and A.A. Elnagar,20083) in aged female fowl.]]>2812651286Mohammadi, H. and Z. Ansari‐Pirsaraei,201498483490McMurtry, J.P. and L.T. Frobish,19816016881688Rezac, P., M. Poschl and Z. Havlicek,200045313317Steinmetz, H.W., R. Vogt, S. Kastner, B. Riond and J.M. Hatt,2007Gallus gallus).]]>19382388Laverty, G., V.S. Elbrond, S.S. Arnason and E. Skadhauge,20061477077Reece, W.O., D.W. Trampel and K.J. Koehler,20068510951097Arnason, S.S., V.S. Elbrond and G. Laverty,2015Lagopus mutus) and the common murre (Uria aalge).]]>1878696Hy-Line International,20162016Zheng, W., M. Shi, S.E. You, H. Ji and D.M. Roesch,200634353Rettenmund, C.L., J.J. Heatley and K.E. Russell,2014Myiopsitta monachus).]]>45256262Reece, W.O., J.L. Sell, D.W. Trampel and W.F. Christensen,20007911201126Rossier, B.C., M.E. Baker and R.A. Studer,201595297340Kellenberger, S. and L. Schild,200282735767Mushtaq, T., M. Sarwar, H. Nawaz, M.A. Mirza and T. Ahmad,20058417161722Schaal, T.P., J. Arango, A. Wolc, J.V. Brady and J.E. Fulton et al.,2016®1 analyzer.]]>95466471Hincke, M.T., Y. Nys, J. Gautron, K. Mann, A.B. Rodriguez-Navarro and M.D. McKee,20121712661280Campbell, T.,20122012pp: 582-597pp: 582-597Irnaten, M., N. Blanchard-Gutton, J. Praetorius and B.J. Harvey,20092+ channels in rat renal cells.]]>74642649Hooge, D.M.,20032003pp: 19Olanrewaju, H.A., J.P. Thaxton, W.A. Dozier III and S.L. Branton,20078613631371