F. Begum
University of Rajshahi, Rajshahi, Bangladesh
M.N. Amin
University of Rajshahi, Rajshahi, Bangladesh
S. Islam
Plant Tissue Culture Section, BCSIR Laboratories, Dhaka, Bangladesh
M.A.K. Azad
Utsunomiya University, Japan
ABSTRACT
Nodal segments of in vitro germinated seedlings of three pummelo varieties [Var.-1 (pulp is pink colour), Var.-2 (pulp is white colour) and Var.-3 (pulp is red colour)] were cultured on half-strength MS medium for axillary shoot proliferation. A large number of shoot buds were produced when such four weeks old culture were subcultured on half-strength MS medium containing 1.0 mg l-1 BAP. Roots were induced when the isolated individual shoots were cultured on half-strength MS medium containing 0.1 mg l-1 each of NAA, IBA or IAA. Cent percent root were observed on half-strength MS medium having 0.1 mg l-1 NAA. These in vitro grown plantlets were then successfully transferred to outside natural condition through successive phases of acclimatization. About 93% of the regenerated plantlets survived under ex vitro condition.
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How to cite this article
F. Begum, M.N. Amin, S. Islam and M.A.K. Azad, 2004. A Comparative Study of Axillary Shoot Proliferation from the Nodal Explants
of Three Varieties Pummelo (Citrus grandis [L.] Osb.). Biotechnology, 3: 56-62.
DOI: 10.3923/biotech.2004.56.62
URL: https://scialert.net/abstract/?doi=biotech.2004.56.62
DOI: 10.3923/biotech.2004.56.62
URL: https://scialert.net/abstract/?doi=biotech.2004.56.62
REFERENCES
- Carimi, F., F. De Pasquale and F.G. Crescimanno, 1999. Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Rep., 11: 935-940.
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