Mohammed A.A. Sarhan
Department of Biological Sciences, King Khalid University, Saudi Arabia
Mustaffa Musa
School Of Medical Sciences, Sains University, Malaysia
Norazmi Mohd Nor
School of Health Science, Sains University, Malaysia
Zainul F. Zainuddin
School of Health Science, Sains University, Malaysia
ABSTRACT
The construction of a synthetic gene coding for the N-terminal of the ice nucleation protein of Pseudomonas syringae using assembly polymerase chain reaction was described. The Inak-n gene was assembled from 34 overlapping oligonucleotides in a single step and amplified by PCR using specific cloning primers. Expression of this gene in E. coli, in the form of a fusion protein, was confirmed by Western blotting. This synthetic gene would be a useful tool for the display of antigens on the surface of bacterial cells.
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How to cite this article
Mohammed A.A. Sarhan, Mustaffa Musa, Norazmi Mohd Nor and Zainul F. Zainuddin, 2005. Synthesis of the N-terminal of the Ice Nucleation Protein Gene of Pseudomonas syringae by Assembly PCR. Biotechnology, 4: 187-193.
DOI: 10.3923/biotech.2005.187.193
URL: https://scialert.net/abstract/?doi=biotech.2005.187.193
DOI: 10.3923/biotech.2005.187.193
URL: https://scialert.net/abstract/?doi=biotech.2005.187.193
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