S.M. Abdul-Awal
Khulna University, Bangladesh
Md. Jahangir Alam
Khulna University, Bangladesh
Md. Raihan Ali
Khulna University, Bangladesh
Md. Nabiul Hasan
Khulna University, Bangladesh
ABSTRACT
In vitro propagation of Trichosanthes dioica Roxb. locally known as patal has been studied. Shoot tips were cultured aseptically on MS media supplemented with different concentrations of cytokinins (BA and 2-ip) and their combinations with NAA, GA3 and CW. The effectiveness of BA was proved to be superior to that of 2-ip alone with respect to shoot proliferation. Combinations of 2-ip with CW and GA3 showed better response than 2-ip alone. The frequency of multiple shoot formation was 100% for shoot tips in MS medium containing BA 1.0 mg L-1 and BA 1.0 mg L-1 + NAA 0.2 mg L-1. A best result with BA was found when explants were cultured on MS medium supplemented with 1.0 mg L-1 BA where the average length was 15.90±0.90 mm. In combinations the highest length was 18.25±0.42 mm at 1.0 mg L-1 2-ip +0.5 mg L-1 GA3 and the highest number of shoots was 3.25±0.22 at 1.0 mg L-1 2-ip+ 10% CW. Efficient rooting was achieved on MS+IBA 0.5 mg L-1. In vitro raised plantlets were then transferred to potted soil.
PDF References
How to cite this article
S.M. Abdul-Awal, Md. Jahangir Alam, Md. Raihan Ali and Md. Nabiul Hasan, 2005. In vitro Propagation of Pointed Gourd (Trichosanthes
dioica Roxb.) from Shoot Tips. Biotechnology, 4: 221-224.
DOI: 10.3923/biotech.2005.221.224
URL: https://scialert.net/abstract/?doi=biotech.2005.221.224
DOI: 10.3923/biotech.2005.221.224
URL: https://scialert.net/abstract/?doi=biotech.2005.221.224
REFERENCES
- Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link