M. Al-Forkan
Department of Botany, University of Chittagong, Chittagong, Bangladesh
M.A. Rahim
Department of Botany, University of Chittagong, Chittagong, Bangladesh
T. Chowdhury
Department of Botany, University of Chittagong, Chittagong, Bangladesh
P. Akter
Department of Botany, University of Chittagong, Chittagong, Bangladesh
L. Khaleda
Department of Botany, University of Chittagong, Chittagong, Bangladesh
ABSTRACT
Embryogenic calli were initiated from mature seed scutellum (MSS) of salt tolerant Indica rice Cvs. BRRI dhan-40, BRRI dhan-41 and Binnatoa on LS 2.5 and MS 2.5 culture media supplemented with 2.5 mg L -1 2, 4-D. Among the Cvs. used, high frequency callusing from mature seed scutellum was observed in Cv. BRRI dhan-40 (74%) on MS 2.5 medium and Cv. BRRI dhan-41 (79%) on LS 2.5 medium, respectively. In terms of plant regeneration, although all Cvs. responded well on the both RM-1 and RM-2 media but the response of different cultivar varied from medium to medium. The highest frequency (69%) of plant regeneration was observed in the variety Binnatoa on RM-1 medium. In contrast, Cv. BRRI dhan-41 produced the highest percentage (79%) of plant whilst plant regeneration frequency was 75% in Cv. Binnatoa on RM-2 regeneration medium, respectively. Embryogenic callus formation and efficient plant regeneration were influenced by culture media and plant genotype.
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How to cite this article
M. Al-Forkan, M.A. Rahim, T. Chowdhury, P. Akter and L. Khaleda, 2005. Development of Highly in vitro Callogenesis and Regeneration System
for Some Salt Tolerant Rice (Oryza sativa L.) Cultivars of Bangladesh. Biotechnology, 4: 230-234.
DOI: 10.3923/biotech.2005.230.234
URL: https://scialert.net/abstract/?doi=biotech.2005.230.234
DOI: 10.3923/biotech.2005.230.234
URL: https://scialert.net/abstract/?doi=biotech.2005.230.234
REFERENCES
- Maas, E.V. and G.J. Hoffman, 1977. Crop salt tolerance-current assessment. J. Irrig. Drainage Div., 103: 115-134.
Direct Link - Linsmaier, E.M. and F. Skoog, 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant., 18: 100-127.
CrossRefDirect Link - Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Rueb, S., M. Leneman, R.A. Schilperoort and L.A.M. Hensgens, 1994. Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell Tissue Org. Cult., 36: 259-264.
CrossRefDirect Link - Xing, X.H., M. Huang and H. Unno, 1996. Shoot regeneration from rice (Oryza sativa L.) callus precultured anaerobically. J. Ferment. Bioengineering, 82: 168-170.
CrossRefDirect Link - Jain, R.K., S. Jain and R. Wu, 1996. Stimulatory effect of water stress on plant regeneration in aromatic Indica rice varieties. Plant Cell Rep., 15: 449-454.
CrossRef - Sun, Z.X., C.Z. Zhao, K.L. Zeng, X.F. Qi and Y.P. Fu, 1983. Somaclonal genetics of rice, Oryza sativa L. Theor. Applied Genet., 67: 67-73.
CrossRefDirect Link - Lee, Y.K., 2001. Microalgal mass culture systems and methods: Their limitation and potential. J. Applied Phycol., 13: 307-315.
CrossRefDirect Link