M. A. Amiza
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R.K. Owusu Apenten
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ABSTRACT
A gastricsin-like proteinase was purified from gastric mucosa of Atlantic cod by a single step purification scheme on ion-exchange of Amberlite CG-50. The purification was very efficient as the recovery was 205% and the purification factor was 1796-fold. The enzyme preparation was homogeneous as observed by SDS-PAGE and isoelectric focusing. The enzyme has an estimated molecular weight of 34 kDa and the pI of 4.4.
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How to cite this article
M. A. Amiza and R.K. Owusu Apenten, 2002. A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua) . Journal of Biological Sciences, 2: 591-595.
DOI: 10.3923/jbs.2002.591.595
URL: https://scialert.net/abstract/?doi=jbs.2002.591.595
DOI: 10.3923/jbs.2002.591.595
URL: https://scialert.net/abstract/?doi=jbs.2002.591.595
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