Masood Akhtar
Department of Veterinary Parasitology, University of Agriculture, Faisalabad, Pakistan
C. S. Hayat
Department of Veterinary Parasitology, University of Agriculture, Faisalabad, Pakistan
ABSTRACT
Protein subunit (PS) and lipopolysaccharides complex subunit (LPCS) antigens were extracted from the in vitro cultured mid gut cells of Boophilus (B.) microplus. PS and LPCS vaccines with and without adjuvant was prepared and injected in buffaloes. Their efficacy was evaluated on the basis of cellular, humoral and challenge responses. There was a significant difference (P<0.05) in cellular, humoral and challenge responses between the two subunit vaccines. The vaccines with adjuvant gave significantly higher cellular, humoral and challenge responses compared to those without adjuvant. The per cent protection and damage of B. microplus in PS vaccines with and without adjuvant was 75.79 and 61.56; and 44.56 and 42.78 per cent, respectively while in LPCS vaccines with and without adjuvant was 89.25 and 73.79; and 76.25 and 64.16 per cent, respectively. The results of the current study confirm the role of protein and carbohydrates as a protective antigen or as a factor for specificity of these antigen.
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How to cite this article
Masood Akhtar and C. S. Hayat, 2001. Protective Efficacy of Subunit Antigens Isolated from In vitro Mid Gut Cells of Boophilus microplus. Pakistan Journal of Biological Sciences, 4: 1165-1169.
DOI: 10.3923/pjbs.2001.1165.1169
URL: https://scialert.net/abstract/?doi=pjbs.2001.1165.1169
DOI: 10.3923/pjbs.2001.1165.1169
URL: https://scialert.net/abstract/?doi=pjbs.2001.1165.1169
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