Wanpen Mesomya
Department of Nutrition and Health, Institute of Food Research and Product Development,Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand
Yaovadee Cuptapun
Department of Nutrition and Health, Institute of Food Research and Product Development,Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand
Duangchan Hengsawadi
Department of Nutrition and Health, Institute of Food Research and Product Development,Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand
Saruda Lohana
Department of Food Processing and Preservation, Institute of Food Research and Product Development, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand
Sompoch Yaieiam
Department of Food Processing and Preservation, Institute of Food Research and Product Development, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand
ABSTRACT
The purpose of this research was to evaluate the effects of germinated soybean on serum lipids by feeding male Sprague-Dawley rats with 6 experimental diets containing 10% protein-casein (control), 10% protein- soy protein isolate (10% SPI), 10% protein-ungerminated soybean (10% UGS), 10% protein- germinated soybean (10% GS), 20% protein-germinated soybean (20% GS) and 28% protein-germinated soybean (28% GS). The diets were prepared from 6 formulas by AOAC, with the following composition:10%, 20% and 28% test protein, 8% oil, 5% water, 5% AIN-mineral, 1% AIN-vitamin, 1% cellulose, 35% sucrose and 35% corn starch. The results showed that serum cholesterol, triglyceride, HDL-c and LDL-c levels were not significantly different among the rats fed with 10% UGS and 10% GS diets. However, serum cholesterol, triglyceride and LDL-c level in rats fed with 20% GS and 28% GS diets were significantly lower than those in rats fed with 10% UGS and 10% GS diets. Serum cholesterol, triglyceride and LDL-c levels in rats fed with 28% GS diet (57.40±6.25, 30.30±5.25,11.80±2.69 mg/dL) were significantly lower than those in rats fed with SPI diet (73.10±13.32, 58.20±13.13, 23.40±5.01 mg/dL). The data indicated that the experimental diet with 28% protein-germinated soybean could reduce serum cholesterol, triglyceride and LDL-c in the experimental rats.
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How to cite this article
Wanpen Mesomya, Yaovadee Cuptapun, Duangchan Hengsawadi, Saruda Lohana and Sompoch Yaieiam, 2013. Effects of Germinated Soybean on Serum Lipids in Rats. Pakistan Journal of Nutrition, 12: 833-836.
DOI: 10.3923/pjn.2013.833.836
URL: https://scialert.net/abstract/?doi=pjn.2013.833.836
DOI: 10.3923/pjn.2013.833.836
URL: https://scialert.net/abstract/?doi=pjn.2013.833.836
REFERENCES
- Abe, T., Y. Kurozumi, W.B. Yao and T. Ubuka, 2008. High-performance liquid chromatographic determination of β-alanine, β-aminoisobutyric acid and γ-aminobutyric acid in tissue extracts and urine of normal and (aminooxy) acetate-treated rats. J. Chromatogr. B., 712: 43-49.
CrossRefDirect Link - Bau, H.M., C. Villaume and L. Mejean, 2000. Effects of soybean (Glycine max) germination on biologically active components, nutritional values of seeds and biological characteristics in rats. Nahrung, 44: 2-6.
PubMed - Bau, H.M., C. Villaume, J.P. Nicolas and L. Mejean, 1997. Effect of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean (Glycine max) seeds. J. Sci. Food Agric., 73: 1-9.
CrossRefDirect Link - Bucolo, G. and H. David, 1973. Quantitative determination of serum triglycerides by the use of enzymes. Clin. Chem., 19: 476-482.
PubMedDirect Link - Carroll, K.K., 1982. Hypercholesterolemia and atherosclerosis: Effects of dietary protein. Fed. Proc., 41: 2792-2796.
PubMed - Carroll, K.K. and E. Kurowska, 1995. Soy consumption and cholesterol reduction: Review of animal and human studies. J. Nutr., 125: 594S-597S.
PubMed - Cho, S.Y., Y.N. Lee and H.J. Park, 2009. Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon. Food Chem., 117: 312-317.
CrossRefDirect Link - Fossati, P. and L. Prencipe, 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28: 2077-2080.
CrossRefPubMedDirect Link - Guo, Y., H. Chen, Y. Song and Z. Gu, 2011. Effects of soaking and aeration treatment on γ-aminobutyric acid accumulation in germinated soybean (Glycine max L.). Food Res. Technol., 232: 787-795.
CrossRefDirect Link - Huang, Y.S., K. Koba, D.F. Horrobin and M. Sugano, 1993. Interrelationship between dietary protein, cholesterol and n-6 polyunsaturated fatty acid metabolism. Prog. Lipid Res., 32: 123-137.
CrossRefDirect Link - Mora-Escobedo, R., M. Robles-Ramirez and E. Ramon-Gallegos, 2009. Effect of protein hydrolysates from germinated soybean on cancerous cells of the human cervix: An in vitro study. Plant Foods Hum. Nutr., 64: 271-278.
CrossRefDirect Link - Mostafa, M.M. and E.H. Rahma, 1987. Chemical and nutritional changes in soybean during germination. Food Chem., 23: 257-275.
CrossRef - Mbithi, S., J. van Camp, R. Rodriguez and A. Huyghebaert, 2001. Effects of sprouting on nutrient and antinutrient composition of kidney beans (Phaseolus vulgaris var. Rose coco). Eur. Food Res. Technol., 212: 188-191.
CrossRef - NCEP, 2001. Third report of the national cholesterol education programme experts panel on detection, evaluation and treatment of high blood cholesterol in adults (ATP III). NIH Publication, National Hearts Lung and Blood Institute, Bethesda, MD., USA. http://www.nhlbi.nih.gov/guidelines/cholesterol/atp3full.pdf.
- Nauck, M., G.R. Wamick and N. Rifai, 2002. Methods for measurement of LDL-cholesterol: A critical assessment of direct measurement by homogeneous assays versus calculation. Clin. Chem., 48: 236-254.
PubMed - Park, M.S.C., B.J. Kudchokar and G.U. Liepa, 1987. Effects of dietary animal and plant proteins on the cholesterol metabolism in immature and mature rats. J. Nutr., 117: 30-35.
PubMedDirect Link - Rimal, A., W. Moon and S.K. Balasubramanian, 2008. Soy food consumption: Effects of perceived product attributes and the food and drug administration allowed health claims. Br. Food J., 110: 607-621.
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