Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field


	Biotechnology
	Year: 2008 \| Volume: 7 \| Issue: 3 \| Page No.: 481-486 DOI: 10.3923/biotech.2008.481.486

Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field

Siti Nurmadihah Ismail, Aishah Mohd Taha, Ng Hong Jing, Roswanira Ab. Wahab, Aidil Abdul Hamid, Rolando V. Pakingking Jr. and Fahrul Huyop

Abstract: A bacterial strain tentatively identified as Pseudomonas sp. R1 was isolated from a paddy (rice) field that could degrade monochloroacetic acid (MCA) for concentrations ranging from 5 to 40 mM. Quantitative agreement between the amount of MCA introduced and chloride released was also found. MCA dehalogenase activity in this strain was found to be inducible. Cell-free extracts displayed dehalogenating activity with specific halogenated organic compound with no activity on dichloropropionic acid or monochloropropionic acid. The estimated Km values for MCA was 0.14 mM. The optimal pH range for MCA dehalogenase activity (between pH 6.5 and 8.0), whereas the thermal stability profile stable up to 50 °C. The results of our current study demonstrated the potential use of Pseudomonas sp. R1 as suitable biological agent for biodegradation of MCA in contaminated agricultural area.

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How to cite this article:

Siti Nurmadihah Ismail, Aishah Mohd Taha, Ng Hong Jing, Roswanira Ab. Wahab, Aidil Abdul Hamid, Rolando V. Pakingking Jr. and Fahrul Huyop, 2008. Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field. Biotechnology, 7: 481-486.

DOI: 10.3923/biotech.2008.481.486

URL: https://scialert.net/abstract/?doi=biotech.2008.481.486

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