This study was aimed at enumerating, isolating and identifying the aerobic mesophilic bacteria associated with poultry faeces obtained from the Obafemi Awolowo University Teaching and Research Farms, Ile-Ife, Nigeria. The second aim was to study the antibiotic sensitivity patterns of the associated bacteria. The aerobic mesophilic bacteria were enumerated, isolated and identified phenotypically following standard microbiological methods. The antibiotic sensitivity patterns of the isolated bacteria against amoxicillin, augmentin, ceftriaxone, chloramphenicol, ciprofloxacin, erythromycin, gentamycin, nitrofurantoin, ofloxacin, pefloxacin, streptomycin, tetracycline, cotrimoxazole were also determined. The total aerobic count of bacteria isolated ranged from 6.15 to 8.64 log cfu g-1 of cockerel faecal sample and 7.18 to 7.67 log cfu g-1 of layer fecal sample. Bacteria associated with the faecal samples were identified as Alcaligenes faecalis, Corynebacterium kutseri, Staphylococcus aureus, Bacillus alvei, Proteus morganii, Corynebacterium ulcerans, Salmonella arizonae, acinetobacter mallei, Staphyloccus sp. Escherichia coli, Aeromonas sp. and Pseudomonas fluorescens. C. kutsceri, C. ulcerans and A. faecalis showed 100% resistance to all the antibiotics tested. Eleven of the isolates showed multiple antibiotics resistance. The quinolones (ofloxacin, ciprofloxacin and pefloxacin) were the most effective of all the antibiotics used. The Multiple Antibiotics Resistance (MAR) index of the bacterial isolates ranged from 0.1 to 1. All the bacterial isolates showed high level (>0.2 MAR index) antibiotics resistance except Aeromonas sp. (2D2) which showed a low-level antibiotics resistance. Using two-way clustered analysis, the relatedness of antibiotics resistance pattern was highest in C. kutsceri and C. ulcerans. The microorganisms isolated from this study are of public health importance and their high level of resistance to commonly used antibiotics in human and veterinary medicine make them a great risk to human and animal.