Omar Shair
King Abdulaziz City for Science and Technology, Institute of Natural Resources and Environmental Research, P.B. Box. 6086. Riyadh 11442 Saudi Arabia
The right and left arms were of the phage were also purified and packaged with packagene. A goat genome library consisting of 2. 1 X 105 phages was constructed and screened by using in situ hybridization technique. Following the procedure of Sambrook et al. (1989) we performed the in-situ hybridization technique. The probe was prepared through Polymerase chain reaction (PCR) using the goat genome as template. The probe was used in the in-situ hybridization technique to screen the library after cloning it into puc 18 vector. Sall and BamHI were used for the analysis of the recombinant phage. The sal 1 fragments were cloned in pGEM5z vector. The partial sequencing of the 4.5kb long Sal 1 fragment cloned in pGEMbz was performed following the procedure of Sambrook et al. (1989). In this study we suggest that the goat 11 casein promoter may be very strong and tissue specific promoter, which may contribute in the progress and development of the mammary gland expression for the production of valuable proteins in the milk.
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How to cite this article
Omar Shair, 1999. The Cloning and Sequencing of the Regulatory Element of Goat Beta-Casein Gene. Pakistan Journal of Biological Sciences, 2: 1236-1239.
DOI: 10.3923/pjbs.1999.1236.1239
URL: https://scialert.net/abstract/?doi=pjbs.1999.1236.1239
DOI: 10.3923/pjbs.1999.1236.1239
URL: https://scialert.net/abstract/?doi=pjbs.1999.1236.1239