|
|
| |
The Prevalence of CagA and CagE Genes in Helicobacter pylori Strains Isolated from Different Patient Groups by Polymerase Chain Reaction |
|
A. Ghasemi,
M.H. Shirazi,
R. Ranjbar,
M.R. Khorramizadeh,
N.E. Daryani and M. Hosseini |
Abstract:
The aim of this study was to investigate the prevalence
of cagA and cagE genes in H. pylori strains isolated
from different patient groups with Non-Ulcer Dyspepsia (NUD), Duodenal
Ulcer (DU), Gastric Ulcer (GU) and Gastric Cancer (GC). The patients admitted
to the gastroenterology unit at Sharyati hospital in Tehran in 2006 were
included in this study. Gastric biopsy specimens were obtained from the
antrum of the stomach from each patient then cultured for detection of
H. pylori. Identification of H. pylori was performed according
to the standard bacteriological methods. Genomic DNA was extracted using
a commercially available Qia gene kit. PCR was done using primers cagA-F,
cagA-R and cagE-F, cagE-R to detect the target genes cagA and cagE,
respectively. Amplified products of target genes were confirmed by sequencing.
The cagA and cagE were detected among 85 and 86% of H.
pylori isolates, respectively. Prevalence of cagA and cagE
genes in the patients with NUD, DU, GU and GC were 22 (64.7%), 28 (100%),
18 (90%), 10 (100%) and 25 (73.5%), 27 (96.4%), 19 (95%), 7 (70%), respectively.
The current study demonstrated a significant correlation between peptic
ulceration and the presence of H. pylori isolates carrying cagE
and cagA genes in Iranian patients.
|
|
|
PDF
Fulltext
XML
References
Citation
Report Citation
|
|
|
How to cite this article:
A. Ghasemi, M.H. Shirazi, R. Ranjbar, M.R. Khorramizadeh, N.E. Daryani and M. Hosseini, 2008. The Prevalence of CagA and CagE Genes in Helicobacter pylori Strains Isolated from Different Patient Groups by Polymerase Chain Reaction. Pakistan Journal of Biological Sciences, 11: 2579-2583.
DOI: 10.3923/pjbs.2008.2579.2583
URL: https://scialert.net/abstract/?doi=pjbs.2008.2579.2583
|
|
|
|
|
| COMMENT ON THIS PAPER |
|
|
|
|
| |
 |
Navigation |
| |
|
|
| |
|
Indexed In |
| |
|
|
Subscribe Today 
