M. Gomathy
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, India
M. Thangaraju
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, India
S. Gunasekaran
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, India
N.O. Gopal
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, India
H. Gopal
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, India
The survival of vegetative and sporulated cells of the Bacillus cultures on the seeds of the crop plants was tried in different combinations. One milliliter inoculum with 1 mL adhesive combination or sterile water showed better results followed by 1.5 mL inoculum with 0.5 mL adhesive or sterile water. The population of 5.5x105 cfu seed-1 on black gram, 10.5x105 cfu seed-1 on soybean and 6.5x105 cfu seed-1 on maize were observed after 12 h of incubation in 1 mL sporulated inoculum mixed with 1 mL of rice gruel. The sporulated inoculum along with rice gruel favoured the adherence of the regenerated cells as rice gruel is rich in nutrient content.
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M. Gomathy, M. Thangaraju, S. Gunasekaran, N.O. Gopal and H. Gopal, 2008. Method and Quantity of Liquid Formulation of Phosphobacteria Required for Seed Inoculation. Pakistan Journal of Biological Sciences, 11: 86-91.
DOI: 10.3923/pjbs.2008.86.91
URL: https://scialert.net/abstract/?doi=pjbs.2008.86.91
DOI: 10.3923/pjbs.2008.86.91
URL: https://scialert.net/abstract/?doi=pjbs.2008.86.91