Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Asian Journal of Plant Sciences
Year: 2008  |  Volume: 7  |  Issue: 5  |  Page No.: 505 - 509

Compared Two Methods for Isolating RNA from Freezing and Nonfreezing Bread Wheat (Triticum aestivum L. ) Plant Tissues

K. Shahrokhabadi, R.T. Afshari, H. Alizade, J.T. Afshari and G.R. Javadi    

Abstract: This report described an improved method for isolating intact purified RNA from freezing organs of bread wheat plants. High-quality RNA is important in studying gene expression. Common RNA extraction protocols have produced poor yields because freezing leaves contain polysaccharides and RNases. We used two methods for isolating RNA and comprised them. CTAB (cetyltrimethylammonium bromide) method protocol is based on a guanidine thiocyanate extraction combined with additional purification steps using butanol and the ionic detergent CTAB. Using this protocol, RNA yields ranged from 40-70 μg of total RNA 200 mg of fresh tissue. This method can be adapted to large-scale isolations, allowing the recovery of larger amounts of intact RNA (up to 250 μg g-1 of fresh tissue).

Cited References   |    Fulltext    |   Related Articles   |   Back
 
 
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility