Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Research Journal of Microbiology
Year: 2014  |  Volume: 9  |  Issue: 3  |  Page No.: 115 - 128

Bacillus subtilis Isolated from Sugarcane Rhizosphere Produces PHA to Defend NaCl Induced Stress

Gargi N. Edkie and D. Theertha Prasad    

Abstract: Bacillus subtilis isolated from sugarcane rhizospheric soil, was found to survive upto 9% NaCl, pH range of 5.5-9.0. It harboured a plasmid of 20 kb which conferred salinity tolerance in transformed E. coli colonies allowing the later to survive 5% NaCl stress. Both Bacillus and transformed E. coli colonies produced PHA, stained positive with nile blue. At low pH and in presence of NaCl, both B. subtilis and plasmid transformed E. coli survived better and produced PHA, but cells did not sustain alkaline pH. Restriction enzyme digested fragments showed homology with genes encoding DHQase1, histidine kinase, dehydratase from Hot_dog family, in Blast search analysis and are found to be associated with PHA synthesis.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility