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Pakistan Journal of Biological Sciences
Year: 2001  |  Volume: 4  |  Issue: 4  |  Page No.: 422 - 424

Inter- and Intraspecific Variations in Wild Rice Species Detected Through Isozyme Markers

N. Iqbal and S. Farooq    

Abstract: Isozyme analyses were used to detect inter- and intraspecific variation in different accessions of wild rice species, 0. officinallis and 0. punctata. Some F1 hybrids between cultivated and wild rice species were also utilized. Fresh leaf extracts were electrophoresed on polyacrylamide gels. Different isozymes viz., Esterases, Glutamate oxaloacetate transaminases and peroxidases were used as biochemical markers. On the basis of isozyme banding profiles, distinct variations were observed between and within the two wild rice species and their different accessions. Based on polymorphic isozyme profiles, hybrids of rice cultivars, Basmati 198 x 0. officinalis, Nonabokra x 0. malampuzhaensis ace: 100957 and EF-6 x 0. malampuzhaensis ace: 100957 were also identified. Our study demonstrated that isozyme markers can successfully be used for detection of genetic diversity as well as for the identification of hybrids.

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