Abstract: Tilapia (Oreochromis niloticus) is one kind of fish are cultured in floating net (KJA) Maninjau lake, West Sumatra. This study was aimed to know about proteolytic bacteria found in the gut of fish Nila can be used as candidate probiotics. Materials used in this study were derived from Nila fish farmers in the vicinity of Maninjau lake. The research method used is the isolation, identification and characterization of bacteria. Identification was conducted on the morphological and biochemical characteristics of bacteria. It was found that 55 isolates of bacteria that are morphological and biochemical grouped into three namely genus are Bacillus, Achromobacter and Enterobacter. Based on the production of extracellular proteolytic enzymes known that isolates B.3.2 had the highest proteolitic index. Based on characteristics examination and PCR analysis the isolate B.3.2 was primarily identified as Bacillus subtilis strain VITNJ1 bacteria can be used as a candidate probiotic.