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Articles by A.H. Gorji
Total Records ( 3 ) for A.H. Gorji
  A.H. Gorji , F. Darvish , M. Esmaeilzadehmoghadam and F. Azizi
  The objective of this study was to provide selection criteria for drought tolerance by RAPD technique. Common wheat (Triticum aestivum L.) is a grass species, cultivated world wide. Genetic diversity evaluation of germplasm is the basis of improvement in wheat. The complexity of the wheat genome has delayed the development and application of molecular markers to this species and wheat now lies behind barley, maize and rice in marker development. However, improvements in marker detection systems and in the techniques used to identify markers linked to useful traits has allowed considerable advances to be made in recent years. The use of PCR based assays having advantage of being quick, easy to use and refractory to many environmental influences can complement traditional methods of germplasm characterization. The RAPD markers were used to determine the genetic differences between the 30 bread wheat genotypes and to determine the molecular markers associated with tolerance to drought.The present study found that RAPD analysis is a valuable diagnostic tool. Different sets of RAPD primer were used to study the polymorphism at molecular level. Initial screens involved growing 20 genotypes at seedling stage under drought conditions (-5 and -8 bar exerted by PEG 6000 in ahydroponic experiment). These tests confirmed the tolerance of the 6 above mentioned genotypes. Thirty 10-mer RAPD primers were used for fingerprinting of the genotypes of which primers P16 (TCGGCGGTTC) and P17 (CTGCATCGTG) produced, respectively a 900 and a 750 bp band present in drought tolerant (absent in others) genotypes. The results substantiate the use of these DNA markers in germplasm screening for drought tolerance in wheat.
  A.H. Gorji and M. Zolnoori
  The aim of this study was to molecularly characterize the thirty-eight wheat cultivars recommended for various regions of Iran and to assess mutual genetic distances, through the use of microsatellite markers. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.19 to 0.78 with a mean of 0.49.Twenty three Polymorphic Microsatellite Markers (PMM) delineated all 34 of the samples, revealing a total of 73 Simple Sequence Repeat (SSR) alleles, i.e., an average of 3.2 alleles per locus. Genetic distances among the 38 genotypes ranged from 0.11 (between genotypes S-83-20 and N-83-3 ) to 0.88 (between genotypes D-83-2 and M-83-5) the mean distance being 0.47. Thirteen groups were obtained by using the unweighted pair-group method with arithmetic means analysis (UPGMA) and fourteen through the Tocher method. Both methods produced similar clusters with one to thirteen genotypes per group. Twenty three polymorphic microsatellite markers delineated all 38 of the samples, revealing a total of 37 simple sequence reapt (ssr) alleles, i.e., an average of 3.1 alleles per locus. The results indicate that these tools may be used to protect intellectual property and for breeding and selection programs.
  A.H. Gorji , Z. Zonoori , M. Zolnoori and A. Jamasbi
  The objective of this study was to evaluate the inheritance of antioxidant activity of triticale under drought stress. For this study, the F1 seeds of a 7x7 half diallel along with their parents were grown in greenhouse in well watered and drought conditions. For this purpose, seven selected lines from breeding programs conducted previously were used as plant entries of the research. Yield and yield components were taken up to determine the best lines to be candidate for future varieties. After exposure of plants to drought stress, Catalase (CAT), the activity of Ascorbate Peroxidase (APX) and Superoxide Dismutase (SOD) enzymes were measured. Presence of over dominance and greater importance of dominance effects in control of traits was observed. All of traits had very high broad sense heritability. In stress condition among the traits APX had high narrow sense heritability (Hn), followed by moderate Hn for CAT and low Hn for SOD and in normal condition APX had highest Hn, followed by SOD with moderate Hn and CAT with low Hn. Regarding to the significant correlations between drought tolerance and antioxidant activity in the literature and considering the highest heritability of antioxidant enzymes in this experiment, it can be inferred that they can be considered as good yardstick for selecting resistance to drought in triticale.
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