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Articles by D. Theertha Prasad
Total Records ( 2 ) for D. Theertha Prasad
  Gargi N. Edkie , Supriya Jadhav and D. Theertha Prasad
  Symbiotic association of rhizospherial organisms helps plants for better adaptability and acquisition of nutrients required for sustainable growth and productivity. Bacillus subtilis isolated from the root rhizosphere of salinity tolerant sugarcane variety, CoM 0265 was evaluated as PGPR using commercially grown cultivar of sugarcane, Co 86032 exposed to salinity stress under greenhouse conditions. Morphological parameters viz., root- shoot length, leaf number, chlorophyll, cations like Mn, Cu, Fe and K showed significant increase with the Bacillus inoculum. MDA, proline, reducing sugar, protein, ascorbate peroxidase and catalase content, minerals like Na and Zn showed an increase with saline stress, but supplementing Bacillus inoculum caused significant reduction in these components and protected plants from salinity stress. The results of the study put together suggest that this Bacillus isolate has potential to be used as bioinoculum in sugarcane cultivation and improve productivity.
  Gargi N. Edkie and D. Theertha Prasad
  Bacillus subtilis isolated from sugarcane rhizospheric soil, was found to survive upto 9% NaCl, pH range of 5.5-9.0. It harboured a plasmid of 20 kb which conferred salinity tolerance in transformed E. coli colonies allowing the later to survive 5% NaCl stress. Both Bacillus and transformed E. coli colonies produced PHA, stained positive with nile blue. At low pH and in presence of NaCl, both B. subtilis and plasmid transformed E. coli survived better and produced PHA, but cells did not sustain alkaline pH. Restriction enzyme digested fragments showed homology with genes encoding DHQase1, histidine kinase, dehydratase from Hot_dog family, in Blast search analysis and are found to be associated with PHA synthesis.
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