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| Articles
by
E. Huq |
Total Records (
5 ) for
E. Huq |
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S.M.A. Kawsar
,
E. Huq
,
N. Nahar
and
Y. Ozeki
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Extracts of Macrotyloma uniflorum plants were
examined as potential sources of phenolic compounds. Reversed phase high
performance liquid chromatography (RP-HPLC) with UV detection was employed
for the identification and quantification of the phenolic acids. Eight
phenolic acids, namely, 3, 4-dihydroxy benzoic, p-hydroxy benzoic,
vanillic, caffeic, p-coumeric, ferulic, syringic and sinapic acids
were isolated from an ethanolic extract of Macrotyloma uniflorum.
The most abundant phenolic acids were p-coumaric acid (8.95 mg
10-2 g of dry sample) and p-hydroxy benzoic acid (7.81
mg/100 g of dry sample). |
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S.M.A. Kawsar
,
G. Mostafa
,
E. Huq
,
N. Nahar
and
Y. Ozeki
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The bioactivity guided separation of the dichloromethane
extract of the aerial parts of Macrotyloma uniflorum Linn. resulted
in the isolation of methyl ester of hexadecanoic and ethyl ester of hexadecanoic
acid mixture (I) and n-hexadecanoic acid (II). The structures of the isolated
compounds were elucidated by spectroscopic analysis, including UV, IR,
1H-NMR, 13C-NMR and mass spectroscopy. In addition,
the fractionated crude extract of 1-butanol exhibited the significant
hemolytic activity by using mouse erythrocytes. |
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S.M.A. Kawsar
,
E. Huq
and
N. Nahar
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The fractionated crude extracts dichloromethane (CH2Cl2),
ethyl acetate (EtOAc), 1-butanol (1-BuOH) and aqueous (H2O)
from aerial parts of Macrotyloma uniflorum were screened for cytotoxicity
using the brine shrimp lethality bioassay technique. Most of the extracts
were found to be non-toxic and this indicates that the ethnobotanical
use (oral applications) of the experimental plant are justified. |
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S. M.A. Kawsar
,
M. Seraj Uddin
,
E. Huq
,
N. Nahar
and
Yasuhiro Ozeki
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In the present research, attempt was taken to explore the antimicrobial potency of the crude extracts of the Macrotyloma uniflorum plant. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the dichloromethane, ethyl acetate, 1-butanol and aqueous extracts ranged from 11-16, 10-24, 10-14 and 10-12 mm, respectively at a concentration of 500 μg disc-1. The ethyl acetate extract showed promising antibacterial activities against all the gram-positive and gram-negative bacteria whereas dichloromethane extract showed moderate activities and the 1-butanol and aqueous extracts did not show any significant antimicrobial activities. In addition, the antifungal activities of all the extractives were tested, using the food poisoning technique. Only dichloromethane extract has been proved to be active against all fungi tested with a higher inhibition activity than standard nystatin. The overall results provide promising baseline information for the potential use of the crude extracts from M. uniflorum in the treatment of bacterial and fungal infections. |
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