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The biosorption of nickel ions on marine Bacillus subtilis N10 free cells and immobilized cells on different porous solid supports has been studied in batch experiments to determine the effect of cell immobilization on metal accumulation. The influence of medium composition on the biosorption activities of these bacterial strains was investigated by the application of Plackett-Burman experimental design. Optimization results represents about 1.2 fold increase in Ni2+ uptake when compared with the control basal culture. Moreover, optimized cultures containing adsorbed cells on pumice particles showed the highest Ni2+ uptake.
