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Articles by Fakher Ayed
Total Records ( 5 ) for Fakher Ayed
  Fakher Ayed , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  Three biological fungicides, Biocont-T, Funga stop and Polyversum constituted, respectively by Trichoderma harzianum, natural extracts (mint oil and citric acid) and Pythium oligandrum, were tested against F. oxysporum f. sp. tuberosi causing potato vascular wilt. Funga stop proved to be the most effective in inhibiting by 72 to 76% the mycelial growth of this pathogen on PDA media after incubation for six days at 25 °C. Biocont-T also limited its development by 37 to 63%. However, Polyversum showed a very little activity in controlling this fungus in vitro. All bio-fungicides reduced disease incidence compared to the untreated control. Funga stop and Biocont-T were the most active during the bioassay. Whereas, Polyversum had a lesser effect in controlling this disease.
  Khaled Hibar , Mejda Daami-Remadi , Fakher Ayed and Mohamed El Mahjoub
  Fusarium oxysporum f. sp. radicis-lycopersici (FORL) is a new emerging pathogen in Tunisia. It causes Fusarium crown and root rot (FCRR) of tomato. Being a new disease, no control methods are available. Therefore, looking for a solution to this pathogen is required. In this study, the efficacy of some chemical fungicides to suppress FORL was evaluated in vitro, in growth chamber as well as under greenhouse conditions. In in vitro tests, all fungicides inhibited mycelial growth of FORL at 75 to 90% with the exception of maneb which entailed the lowest growth inhibition estimated at 40%. Under growth chamber trials, with the exception of benomyl, the other tested fungicides (Hymexazol, Hydroxyquinolin, Sodium Tetraborohydrate Decahydrate, Oxyquinolin and Flutriafol+Thiabendazole) have significantly reduced disease incidence. Under greenhouse conditions, results were more encouraging. Indeed, the use of Hymexazol reduces the percentage of dead plants at 6.4%. This study demonstrated the efficacy of some chemical fungicides in controlling FORL especially when they were incorporated to the culture substrate.
  Fakher Ayed , Mejda Daami-Remadi , Hager Jebari , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  In 2005, a crown and root rot, a yellowing and a death of muskmelon plants were observed in some tunisian melon-growing regions. Isolation was made from diseased tissues. Twelve isolates of Fusarium solani were identified and used to inoculate melon-seedlings by the root dip method for 1 min. These 12 isolates caused typical symptoms of Fusarium crown rot and their pathogenicity indicated that they are identical to Fusarium solani f. sp. cucurbitae. Disease severity on melon-plants, cv. “Ananas d’Amérique”, was estimated by indexes of the leaf damage and crown rot. Analysis of variance revealed significant difference between control and inoculated plants. A significant difference was also observed between some F. solani f. sp. cucurbitae isolates in causing leaf damage and crown rot and a negative effect was observed mainly on plant fresh weight revealing impact of this pathogen on muskmelon growth.
  Fakher Ayed , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine , Khaled Hibar and Mohamed El Mahjoub
  Four fungicides, which active ingredients are hymexazol, azoxystrobin, fludioxonil and quinoline, are tested in vitro and in vivo against five isolates of Fusarium oxysporum f. sp. tuberosi, causal agent of potato Fusarium wilt. Pathogen radial growth noted on PDA amended with fungicides after incubation for four days at 25°C varied upon pathogen isolates. Mycelial growth of all pathogen isolates was significantly inhibited by fungicide PDA amendment; inhibition obtained by hymexazol treatment reached 77% comparatively to untreated control. All chemical fungicides reduced disease incidence compared to the untreated control. Hymexazol and azoxystrobin are the most active during all the bioassay period. Whereas, fludioxonil and quinoline showed a limited effect in controlling Fusarium wilt development.
  Fakher Ayed , Mejda Daami- Remadi , Hayfa Jabnoun- Khiareddine and Mohamed El Mahjoub
  Pathogen isolations from potato tubers showing dry rot symptoms revealed the presence of Fusarium oxysporum f. sp. tuberosi in different Tunisian regions. Pathogenicity tests of different isolates were realized on potato plants. Typical symptoms of vascular wilt disease were observed and noted. After wilting, inoculated plants were totally damaged. Trichoderma spp. were evaluated for their antagonistic potential against F. oxysporum f. sp. tuberosi in vitro and in vivo. Trichoderma harzianum, T. viride and T. virens inhibited the mycelial growth of F. oxysporum f. sp. tuberosi. The antagonism included lysis and dissolution of the host cytoplasm and/or transformation into cords and/or coiling around pathogen hyphae. Moreover, substrate application of Trichoderma species (108 spores per mL) before inoculation by F. oxysporum f. sp. tuberosi controlled Fusarium wilt of potato plants compared with non-inoculated plants and untreated-inoculated plants. This approach may be beneficial for biological control in F. oxysporum f. sp. tuberosi and could allow protecting plants from this pathogen.
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