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The bioactivity guided separation of the dichloromethane
extract of the aerial parts of Macrotyloma uniflorum Linn. resulted
in the isolation of methyl ester of hexadecanoic and ethyl ester of hexadecanoic
acid mixture (I) and n-hexadecanoic acid (II). The structures of the isolated
compounds were elucidated by spectroscopic analysis, including UV, IR,
1H-NMR, 13C-NMR and mass spectroscopy. In addition,
the fractionated crude extract of 1-butanol exhibited the significant
hemolytic activity by using mouse erythrocytes.
