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Articles by Gargi N. Edkie
Total Records ( 3 ) for Gargi N. Edkie
  Gargi N. Edkie , Supriya Jadhav and D. Theertha Prasad
  Symbiotic association of rhizospherial organisms helps plants for better adaptability and acquisition of nutrients required for sustainable growth and productivity. Bacillus subtilis isolated from the root rhizosphere of salinity tolerant sugarcane variety, CoM 0265 was evaluated as PGPR using commercially grown cultivar of sugarcane, Co 86032 exposed to salinity stress under greenhouse conditions. Morphological parameters viz., root- shoot length, leaf number, chlorophyll, cations like Mn, Cu, Fe and K showed significant increase with the Bacillus inoculum. MDA, proline, reducing sugar, protein, ascorbate peroxidase and catalase content, minerals like Na and Zn showed an increase with saline stress, but supplementing Bacillus inoculum caused significant reduction in these components and protected plants from salinity stress. The results of the study put together suggest that this Bacillus isolate has potential to be used as bioinoculum in sugarcane cultivation and improve productivity.
  Gargi N. Edkie and D. Theertha Prasad
  PHA synthase IIIC gene of 1114 bp isolated from Bacillus is AT rich. Scratch Protein Predictor analysis suggest that the protein is globular in nature and predicted 5 disulphide bridges. GRAVY index suggests that the protein is hydrophobic in nature. SOPMA analysis indicate that the predicted protein possess 44.71% alpha-helices with transmembrane domains, possibly play a role in cell recognition and signalling. Prediction of the position of helices using EXPASY tool suggest that Bacillus PHA synthase IIIC has two major helices with lengthy NORS region. Prosite- prediction analysis suggests that Bacillus PHA synthase IIIC has potential N-glycosylation, myristylation, protein kinase C and casein kinase II type phosphorylation domains. These are responsible for general metabolic activities associated with the protein and most of them are located in NORS region. Sequence alignment of PHA synthase IIIC using ClustalW program and MEGA4.0 program showed 96% identity with Bacillus megaterium PHA synthase IIIC gene.
  Gargi N. Edkie and D. Theertha Prasad
  Bacillus subtilis isolated from sugarcane rhizospheric soil, was found to survive upto 9% NaCl, pH range of 5.5-9.0. It harboured a plasmid of 20 kb which conferred salinity tolerance in transformed E. coli colonies allowing the later to survive 5% NaCl stress. Both Bacillus and transformed E. coli colonies produced PHA, stained positive with nile blue. At low pH and in presence of NaCl, both B. subtilis and plasmid transformed E. coli survived better and produced PHA, but cells did not sustain alkaline pH. Restriction enzyme digested fragments showed homology with genes encoding DHQase1, histidine kinase, dehydratase from Hot_dog family, in Blast search analysis and are found to be associated with PHA synthesis.
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