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by
Gargi N. Edkie |
Total Records (
3 ) for
Gargi N. Edkie |
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Gargi N. Edkie
,
Supriya Jadhav
and
D. Theertha Prasad
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Symbiotic association of rhizospherial organisms helps plants for better adaptability and acquisition of nutrients required for sustainable growth and productivity. Bacillus subtilis isolated from the root rhizosphere of salinity tolerant sugarcane variety, CoM 0265 was evaluated as PGPR using commercially grown cultivar of sugarcane, Co 86032 exposed to salinity stress under greenhouse conditions. Morphological parameters viz., root- shoot length, leaf number, chlorophyll, cations like Mn, Cu, Fe and K showed significant increase with the Bacillus inoculum. MDA, proline, reducing sugar, protein, ascorbate peroxidase and catalase content, minerals like Na and Zn showed an increase with saline stress, but supplementing Bacillus inoculum caused significant reduction in these components and protected plants from salinity stress. The results of the study put together suggest that this Bacillus isolate has potential to be used as bioinoculum in sugarcane cultivation and improve productivity. |
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Gargi N. Edkie
and
D. Theertha Prasad
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PHA synthase IIIC gene of 1114 bp isolated from Bacillus
is AT rich. Scratch Protein Predictor analysis suggest that the protein is globular
in nature and predicted 5 disulphide bridges. GRAVY index suggests that the
protein is hydrophobic in nature. SOPMA analysis indicate that the predicted
protein possess 44.71% alpha-helices with transmembrane domains, possibly play
a role in cell recognition and signalling. Prediction of the position of helices
using EXPASY tool suggest that Bacillus PHA synthase IIIC has two major
helices with lengthy NORS region. Prosite- prediction analysis suggests that
Bacillus PHA synthase IIIC has potential N-glycosylation, myristylation,
protein kinase C and casein kinase II type phosphorylation domains. These are
responsible for general metabolic activities associated with the protein and
most of them are located in NORS region. Sequence alignment of PHA synthase
IIIC using ClustalW program and MEGA4.0 program showed 96% identity with Bacillus
megaterium PHA synthase IIIC gene. |
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Gargi N. Edkie
and
D. Theertha Prasad
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Bacillus subtilis isolated from sugarcane rhizospheric soil, was found
to survive upto 9% NaCl, pH range of 5.5-9.0. It harboured a plasmid of 20 kb
which conferred salinity tolerance in transformed E. coli colonies allowing
the later to survive 5% NaCl stress. Both Bacillus and transformed
E. coli colonies produced PHA, stained positive with nile blue. At low pH
and in presence of NaCl, both B. subtilis and plasmid transformed E.
coli survived better and produced PHA, but cells did not sustain alkaline
pH. Restriction enzyme digested fragments showed homology with genes encoding
DHQase1, histidine kinase, dehydratase from Hot_dog family, in Blast search
analysis and are found to be associated with PHA synthesis. |
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