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Articles by H. Jabnoun-Khiareddine
Total Records ( 6 ) for H. Jabnoun-Khiareddine
  M. Daami-Remadi , K. Hibar , H. Jabnoun-Khiareddine , F. Ayed and M. El Mahjoub
  Trichoderma harzianum and T. viride were evaluated for their antagonistic activity against F. oxysporum f.sp. tuberosi, F. solani, F. graminearum and F. sambucinum causing potato dry rot in Tunisia. In vitro dual culture experiments, observed after incubation at 25°C for 6 days, showed that both tested Trichoderma species significantly reduced mycelial growth of Fusarium spp. comparatively to untreated controls and that a significant interaction was noted between both fixed factors (at p≤0.05). Light microscopic studies of antagonists x Fusarium spp. in vitro interactions showed lesser mycelium density, severe lysis, lesser pathogen sporulation, mycelial cords formation and early chlamydospores induction which were observed only at the confrontation zone of both microorganisms. Potato tubers, cv. Spunta, individually treated, at inoculation sites by 100 μL of Trichoderma spp. suspensions (108 spores mL-1), 24 h prior inoculation by Fusarium species, showed reduction in dry rot development, after 21 days of incubation at 25-27°C, comparatively to untreated controls.
  M. Daami-Remadi , F. Ayed , H. Jabnoun-Khiareddine , K. Hibar and M. El Mahjoub
  Several fungicides are tested individually or in dual combination against four Fusarium species causing potato tuber dry rot in Tunisia. Incorporated into the culture media PDA, the tested fungicides significantly inhibited the mycelial growth of all Fusarium species incubated at 25°C for 6 days; a significant interaction was observed between the both fixed factors (p≤0.05). Applied on potato tubers (tuber immersion for 10 min) prior inoculation, all tested fungicides, combined or not, have significantly reduced by more than 50%, comparatively to the untreated controls, the development of dry rot occasioned by Fusarium sp. after 21 days of incubation at 25-27°C. A significant interaction was observed between the treatments and the Fusarium species (p≤0.05). In natural conditions, tuber treatment by the tested fungicides, prior their definitive storage, has reduced dry rot development by about 50%. A synergistic effect was observed in vitro, in vivo and in situ between the mixed fungicides traduced by a better efficacy, in comparison to their individual effects, showing their compatibility and the promotion of the disease control. These combined fungicides could play a role in an integrated pest management against potato tuber-borne pathogens.
  M. Daami-Remadi , H. Jabnoun-Khiareddine , F. Ayed and M. El Mahjoub
  Mycelial growth on PDA of Fusarium graminearum, F. oxysporum f. sp. tuberosi, F. sambucinum and F. solani, causal agents of potato tuber dry rot, depends upon temperatures ranging from 5 to 40°C. An interaction between pathogens tested and temperatures used for culture incubation was observed (at p≤0.05). These Fusarium species showed in vivo both thermal pics of aggressivity: a first one at low temperatures (10-15°C) and a second at higher temperatures (30-35°C). Nevertheless, at temperatures less than 25°C, F. sambucinum and F. graminearum were the most aggressive, while F. solani was the most aggressive at temperatures superior to 30°C.
  F. Ayed , M. Daami-Remadi , H. Jabnoun-Khiareddine and M. El Mahjoub
  Behaviour of some potato cultivars to Fusarium wilt caused by Fusarium oxysporum f.sp. tuberosi was studied. Plants were inoculated after two weeks by irrigation with a conidial suspension (107 spores mL-1). Variations of Fusarium wilt incidence were observed on potato 14 cultivars. Cv. Baraka was the most tolerant, Lyra and Platina were the most susceptible and Asterix, Alaska, Safrane and Timate were found to be intermediate with various degrees of response. Pathogen transmission to progeny tubers was assessed by planting daughter tubers coming from inoculated and non inoculated plants. Differences in incidence of cultivar susceptibility were significant. Disease incidence of Latona and Timate was the most and the least important, respectively.
  M. Daami-Remadi , H. Jabnoun-Khiareddine , F. Ayed , K. Hibar , I.E.A Znaidi and M. El Mahjoub
  Eight thermo-resistant fungi isolated from compost extracts are tested, for the first time in Tunisia, for their antagonistic activity, in vitro by dual culture with Fusarium spp. on PDA and in vivo by wound treatment prior to tuber inoculation. In vitro experiments showed that all tested compost fungi significantly inhibited mycelial growth of F. graminearum, F. sambucinum, F. solani and F. oxysporum f.sp. tuberosi, observed after incubation at 25°C during 6 days, revealing a higher competition. Light microscopic studies of tested pathogen mycelium, removed from the confrontation zone of both microorganisms, showed multiple mechanisms of action including mycoparasitism, lysis, early formation of chlamydospores and induction of mycelial cords via anatomosis between mycelial filaments. Tuber (cv. Spunta) treatment, 24 h before their inoculation by individually thermoresistant fungi suspensions, reduced development of dry rot after incubation for 21 days at 25-27°C, comparatively to the untreated control and this for the majority of the Fusarium complex tested.
  M. Daami-Remadi , H. Jabnoun-Khiareddine , F. Ayed and M. El Mahjoub
  Several fungicides were tested against some isolates belonging to four Fusarium species causing potato tuber dry rot in Tunisia. Incorporated into the culture media PDA, the tested fungicides significantly inhibited the mycelial growth, observed after incubation at 25°C for 4 days, of all Fusarium isolates including those of F. sambucinum resistant to benzimidazoles. A significant interaction (p ≤ 0.05) was observed between both fixed factors where inhibition percentage varied depending on tested pathogens and fungicides. Applied on potato tubers (tuber immersion for 10 min) prior inoculation, certain tested fungicides such as azoxystrobin and fludioxonil significantly reduced by more than 50%, comparatively to the untreated controls, the development of dry rot occasioned by F. graminearum and F. sambucinum observed after 21 days of incubation at 25-27°C. A significant interaction (p ≤ 0.05) was noted between the treatments and the Fusarium species traduced by a variable inhibition percentage depending on tested pathogen and fungicides.
 
 
 
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