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Articles by Irda Fidrianny
Total Records ( 5 ) for Irda Fidrianny
  Irda Fidrianny , Fadhila Syifa and Muhamad Insanu
  Background and Objectives: Antioxidants are able to stabilize or eliminate free radicals before they attack the cells. Antioxidant compounds such as flavonoid substances are essential for maintaining optimum cellular work. Phenol and flavonoid compounds are widely contained in plants, included in strawberry. The purposes of this research were to compare antioxidant profiles from different organs of strawberry using two antioxidant testing methods 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric reducing antioxidant capacities (CUPRAC) and also its phytochemical content. Materials and Methods: Antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC) were conducted by UV-Vis spectrophotometry. Correlation of TPC and TFC with their IC50 of DPPH and EC50 CUPRAC were analyzed by Pearson’s method. Results: All different organs extracts of strawberry exposed IC50 of DPPH varied from 0.22-10.14 μg mL–1 and EC50 of CUPRAC from 130.42-250.14 μg mL–1. Ethanol stem extract gave the highest TPC 18.62 g gallic acid equivalent (GAE) 100 g–1, while ethyl acetate leaves extract showed the highest TFC 7.40 g quercetin equivalent (QE) 100 g–1. The TPC in fruit and leaves extract were significantly negative correlation with their IC50 DPPH (r = -0.865; r = -0.970, p<0.01). Conclusion: All different organs extracts of strawberry were very strong antioxidant by DPPH method. Waste products of strawberry (leaves and stem) had antioxidant potential. The major contributor in antioxidant activities of fruit and leaves extracts by DPPH assay were phenolic compounds. Only strawberry fruits extract showed linear results in DPPH and CUPRAC assays.
  Lia Amalia , Kusnandar Anggadiredja , Sukrasno , Irda Fidrianny and Rini Inggriani
  The wild cosmos (Cosmos caudatus Kunth, Asteraceae) is one of the vegetables mostly consumed by rural people of Central and East Java Provinces of Indonesia and those of the Malay Peninsula. Yet, it has not been widely used medicinally. The leaf has distinctive taste as well as odor and contains high level of flavonoid, especially flavonol and flavon glycosides which have potent antioxidant activity. In this study, in an attempt to explore the antihypertensive effect, wild cosmos leaf aqueous extract was tested in rats treated with adrenaline and sodium chloride. The frequency of heart rate and amplitude of stroke volume were measured using the non-invasive tail cuff method. The extract at doses of 500 and 1000 mg kg-1 showed similar potency to that of 9 mg kg-1 atenonol, in lowering both parameters, induced by adrenaline. However, after sodium chloride, the extract only suppressed the amplitude and this effect was comparable to that of 0.45 mg kg-1 hydrochlorothiazide and 13.5 mg kg-1 captopril. Besides cardiac effects, the extract also demonstrated diuretic activity which was comparable to that of 1.8 mg kg-1 furosemide. Taken together, results of present study suggest that wild cosmos leaf extract have antihypertensive effect which may be related, at least in part, to the decreased cardiac output and induction of diuresis. The results may further indicate that consumption of wild cosmos leaf in diet is beneficial for hypertensive patients.
  Elin Yulinah Sukandar , Nethiyakalyani Sunderam and Irda Fidrianny
  Temu kunci (Kaempferia pandurata (Roxb.)) has a number of benefits and one of these is antibacterial. The rhizome is said to have antibacterial activity against Streptococcus mutans, Lactocillus sp. and Candida albicans. The aim of the study is to test the antibacterial activity of Kaempferia pandurata (Roxb.) rhizome ethanol extract on methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant coagulase negative Staphylococci (MRCNS), methicillin-sensitive Staphylococcus aureus (MSSA), Bacillus subtilis and Salmonella typhi. Antimicrobial activity of the extract was assayed by the microdilution method using Mueller Hinton Broth with sterilized 96 round-bottomed microwells to determine the Minimum Inhibitory Concentration (MIC) as well as to determine the time-kill activity. The MIC of the extract was 16 ppm for both Bacillus subtilis and MRSA; 8 ppm for both MSSA and Salmonella typhi and 4 ppm for MRCNS. Ethanol extract of Kaempferia pandurata (Roxb.) showed antibacterial activity against all the tested bacteria and was the most potent against MRCNS, with MIC 4 ppm. The killing profile test of the extract displayed bactericidal activity at 8-16 ppm against MRSA, MSSA, Bacillus subtilis and Salmonella typhi and bacteriostatic activity at 4 ppm towards MRCNS.
  Sukrasno , Kartika , Irda Fidrianny , Elfahmi and Khairul Anam
  Curcuma xanthorrhiza is one the most important of Indonesian crude drug. Its pharmacological activities are usually associated with curcuminoid and the essential oil content. This study was aimed at studying the influence of the crude drug preparation on the content and the composition of volatile oil Curcuma rhizome. The bulky rhizomes were stored at room temperatures and their volatile oil contents measured. The composition of the oil was analyzed by GC-MS. The rhizomes were sliced, dried, comminuted and their oil content measured. Storage of bulky rhizome at room temperature continuously decreased the yield of volatile oil from bulky fresh rhizome. Storage did not change the number and the identity of the oil component: However, it altered their composition. Germacron, xanthorrhizol and α-curcumene increased while dipi-α-cedren decreased during storage. Upon slicing, drying under the sun, drying in an air oven, grinding and storage of the dried Curcuma rhizome, most of the volatile oil was still retained in the crude drug. Storage of bulky Curcuma rhizome continuously decreased its volatile oil content and the lost of oil reach 57% after 12 weeks. It is suggested that to assure the highest level of volatile oil content in the preparation of Curcuma crude drug, the rhizome should be immediately sliced and dried after harvest.
  Irda Fidrianny , Ari Sri Windyaswari and Komar Ruslan Wirasutisna
  Antioxidant is a molecule that has ability to inhibit or prevent the other molecules being oxidized. Natural antioxidant is a compound which in limited number can inhibit or prevent the other molecules being oxidized. Sweet potatoes (Ipomoea batatas L. Lam) leaves extract had antioxidant activities. The aim of this research, were to determine antioxidant capacity of various leaves extract from five colors varieties of sweet potatoes tubers using two methods of antioxidant testing and its correlation with total flavonoid, phenolic and carotenoid content. The research was started with reflux extraction using gradient polarity solvent (n-hexane, ethyl acetate and ethanol). Antioxidant capacities of each extracts was performed by DPPH (1,1-diphenyl-2-picrylhidrazyl) and ABTS (2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)) diammonium salt. Determination of total flavonoid, phenolic and carotenoid content was done by UV-Visible spectrophotometry. The highest ABTS capacity was given by sample RY3 and the highest DPPH scavenging activity by sample RP3. The total phenolic contents in sample RP was the highest significantly correlated with ABTS and DPPH scavenging capacities. The ABTS scavenging activity in sample Y had positively high correlation with total flavonoid contents. The total carotenoid contents in sample O had high correlation with ABTS scavenging activity and sample Y with DPPH scavenging capacity. There was no significant correlation between ABTS scavenging capacities and DPPH scavenging capacities of various leaves extract from five colors varieties of sweet potatoes tubers.
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