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Articles by K. Hibar
Total Records ( 3 ) for K. Hibar
  M. Daami-Remadi , K. Hibar , H. Jabnoun-Khiareddine , F. Ayed and M. El Mahjoub
  Trichoderma harzianum and T. viride were evaluated for their antagonistic activity against F. oxysporum f.sp. tuberosi, F. solani, F. graminearum and F. sambucinum causing potato dry rot in Tunisia. In vitro dual culture experiments, observed after incubation at 25°C for 6 days, showed that both tested Trichoderma species significantly reduced mycelial growth of Fusarium spp. comparatively to untreated controls and that a significant interaction was noted between both fixed factors (at p≤0.05). Light microscopic studies of antagonists x Fusarium spp. in vitro interactions showed lesser mycelium density, severe lysis, lesser pathogen sporulation, mycelial cords formation and early chlamydospores induction which were observed only at the confrontation zone of both microorganisms. Potato tubers, cv. Spunta, individually treated, at inoculation sites by 100 μL of Trichoderma spp. suspensions (108 spores mL-1), 24 h prior inoculation by Fusarium species, showed reduction in dry rot development, after 21 days of incubation at 25-27°C, comparatively to untreated controls.
  M. Daami-Remadi , F. Ayed , H. Jabnoun-Khiareddine , K. Hibar and M. El Mahjoub
  Several fungicides are tested individually or in dual combination against four Fusarium species causing potato tuber dry rot in Tunisia. Incorporated into the culture media PDA, the tested fungicides significantly inhibited the mycelial growth of all Fusarium species incubated at 25°C for 6 days; a significant interaction was observed between the both fixed factors (p≤0.05). Applied on potato tubers (tuber immersion for 10 min) prior inoculation, all tested fungicides, combined or not, have significantly reduced by more than 50%, comparatively to the untreated controls, the development of dry rot occasioned by Fusarium sp. after 21 days of incubation at 25-27°C. A significant interaction was observed between the treatments and the Fusarium species (p≤0.05). In natural conditions, tuber treatment by the tested fungicides, prior their definitive storage, has reduced dry rot development by about 50%. A synergistic effect was observed in vitro, in vivo and in situ between the mixed fungicides traduced by a better efficacy, in comparison to their individual effects, showing their compatibility and the promotion of the disease control. These combined fungicides could play a role in an integrated pest management against potato tuber-borne pathogens.
  M. Daami-Remadi , H. Jabnoun-Khiareddine , F. Ayed , K. Hibar , I.E.A Znaidi and M. El Mahjoub
  Eight thermo-resistant fungi isolated from compost extracts are tested, for the first time in Tunisia, for their antagonistic activity, in vitro by dual culture with Fusarium spp. on PDA and in vivo by wound treatment prior to tuber inoculation. In vitro experiments showed that all tested compost fungi significantly inhibited mycelial growth of F. graminearum, F. sambucinum, F. solani and F. oxysporum f.sp. tuberosi, observed after incubation at 25°C during 6 days, revealing a higher competition. Light microscopic studies of tested pathogen mycelium, removed from the confrontation zone of both microorganisms, showed multiple mechanisms of action including mycoparasitism, lysis, early formation of chlamydospores and induction of mycelial cords via anatomosis between mycelial filaments. Tuber (cv. Spunta) treatment, 24 h before their inoculation by individually thermoresistant fungi suspensions, reduced development of dry rot after incubation for 21 days at 25-27°C, comparatively to the untreated control and this for the majority of the Fusarium complex tested.
 
 
 
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