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Articles by Khaled Hibar
Total Records ( 3 ) for Khaled Hibar
  Fakher Ayed , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine , Khaled Hibar and Mohamed El Mahjoub
  Four fungicides, which active ingredients are hymexazol, azoxystrobin, fludioxonil and quinoline, are tested in vitro and in vivo against five isolates of Fusarium oxysporum f. sp. tuberosi, causal agent of potato Fusarium wilt. Pathogen radial growth noted on PDA amended with fungicides after incubation for four days at 25°C varied upon pathogen isolates. Mycelial growth of all pathogen isolates was significantly inhibited by fungicide PDA amendment; inhibition obtained by hymexazol treatment reached 77% comparatively to untreated control. All chemical fungicides reduced disease incidence compared to the untreated control. Hymexazol and azoxystrobin are the most active during all the bioassay period. Whereas, fludioxonil and quinoline showed a limited effect in controlling Fusarium wilt development.
  Hayfa Jabnoun-Khiareddine , Mejda Daami-Remadi , Khaled Hibar , Fakher Ayed and Mohamed El Mahjoub
  In many surveys in the Tunisian Sahel, several isolates of Verticillium have been collected from vegetable crops showing symptoms of Verticillium wilt. These isolates were identified as V. dahliae, V. albo-atrum and V. tricorpus on the basis of their morphological and cultural characteristics. Four isolates of V. dahliae, two isolates of V. albo-atrum and two isolates of V. tricorpus were examined for their mycelial growth at different incubation temperatures and their virulence against tomato and eggplant cultivars. Seedlings at the three leaf stage were root-dipped in a conidial suspension (107 conidia mL-1) and maintained in the glasshouse at 21-25°C. Disease severity was assessed through the index of leaf damage and plant height, recorded eight weeks after inoculation. Growth test showed that the majority of Verticillium isolates were able to grow from 10 to 30°C. All tomato and eggplant seedlings exhibited typical Verticillium symptoms compared to the non-inoculated control. The index of leaf damage, as well as plant height, differs significantly depending on cultivars and isolates. V. albo-atrum and V. tricorpus were moderately to highly pathogenic against eggplant and tomato plants. V. dahliae was the most aggressive against these hosts, showing a high level of pathogenicity to both eggplant and tomato plants.
  Mejda Daami-Remadi , Fakher Ayed , Hayfa Jabnoun-Khiareddine , Khaled Hibar and Mohamed El Mahjoub
  Four Bacillus sp. isolates were individually essayed against Fusarium solani, F. oxysporum f.sp. tuberosi, F. graminearum and F. sambucinum following an in vitro dual culture plate technique and in vivo pre-inoculation tuber treatment. All tested bacterial isolates significantly reduced radial mycelial growth of Fusarium spp., on PDA after 3 days of incubation at 25°C, comparatively to the untreated controls. Light microscopic studies of antagonist x Fusarium spp. in vitro interaction showed several hyphal abnomalies traduced particularly by lesser mycelium density, severe hyphal lysis, lesser sporulation, mycelial cords formation and early chlamydospores induction observed only at the confrontation zone. Potato tubers, cv. Spunta, treated by Bacillus sp. 24 h before individually inoculation by Fusarium species, showed reduced dry rot development after 21 days of incubation at 25-27°C comparatively to untreated controls.
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